Anti-IL1 beta抗体[EP407Y] (ab45692)


  • 产品名称Anti-IL1 beta抗体[EP407Y]
    参阅全部 IL1 beta 一抗
  • 描述
    兔单克隆抗体[EP407Y] to IL1 beta
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant human IL-1 beta protein.

  • 常规说明

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.



Our Abpromise guarantee covers the use of ab45692 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
  • 应用说明WB: 1/20000. (approximately 0.05ug/lane [0.5ug/200ul] of recombinant protein was loaded on the membrane). Detects a band of approximately 17 kDa (predicted molecular weight: 30 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells.
    • 组织特异性Expressed in activated monocytes/macrophages (at protein level).
    • 序列相似性Belongs to the IL-1 family.
    • 翻译后修饰Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated.
    • 细胞定位Cytoplasm, cytosol. Lysosome. Secreted, exosome. Cytoplasmic vesicle, autophagosome. Secreted. The precursor is cytosolic. In response to inflammasome-activating signals, such as ATP for NLRP3 inflammasome or bacterial flagellin for NLRC4 inflammasome, cleaved and secreted. IL1B lacks any known signal sequence and the pathway(s) of its secretion is(are) not yet fully understood (PubMed:24201029). On the basis of experimental results, several unconventional secretion mechanisms have been proposed. 1. Secretion via secretory lysosomes: a fraction of CASP1 and IL1B precursor may be incorporated, by a yet undefined mechanism, into secretory lysosomes that undergo Ca(2+)-dependent exocytosis with release of mature IL1B (PubMed:15192144). 2. Secretory autophagy: IL1B-containing autophagosomes may fuse with endosomes or multivesicular bodies (MVBs) and then merge with the plasma membrane releasing soluble IL1B or IL1B-containing exosomes (PubMed:24201029). However, autophagy impacts IL1B production at several levels and its role in secretion is still controversial. 3. Secretion via exosomes: ATP-activation of P2RX7 leads to the formation of MVBs containing exosomes with entrapped IL1B, CASP1 and other inflammasome components. These MVBs undergo exocytosis with the release of exosomes. The release of soluble IL1B occurs after the lysis of exosome membranes (By similarity). 4. Secretion by microvesicle shedding: activation of the ATP receptor P2RX7 may induce an immediate shedding of membrane-derived microvesicles containing IL1B and possibly inflammasome components. The cytokine is then released in the extracellular compartment after microvesicle lysis (PubMed:11728343). 5. Release by translocation through permeabilized plasma membrane. This may occur in cells undergoing pyroptosis due to sustained activation of the inflammasome (By similarity). These mechanisms may not be not mutually exclusive.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Catabolin antibody
      • H1 antibody
      • IL 1 antibody
      • IL 1 beta antibody
      • IL-1 beta antibody
      • IL1 BETA antibody
      • IL1B antibody
      • IL1B_HUMAN antibody
      • IL1F2 antibody
      • Interleukin 1 beta antibody
      • Interleukin-1 beta antibody
      • OAF antibody
      • OTTHUMP00000162031 antibody
      • Preinterleukin 1 beta antibody
      • Pro interleukin 1 beta antibody
      see all

    Anti-IL1 beta antibody [EP407Y] 图像

    Anti-IL1 beta antibody [EP407Y] (ab45692)参考文献

    ab45692 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (Tonsil, Lung)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Diva Decloaker
    Permeabilization No
    Specification Tonsil, Lung
    Blocking step DAKO Protein block, serum-free as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.25% · Temperature: 25°C
    Fixative Formalin

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    提交于 Apr 13 2016