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Full length protein aa 118-269. Full length mature protein minus the propeptide from aa 1-117.
Database link: P10749
Our Abpromise guarantee covers the use of ab9722 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution. To detect mIL-1b by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-1b is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.|
|ELISA||Use at an assay dependent dilution. To detect mIL-1b by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mIL-1b.|
|Neutralising||Use at an assay dependent dilution. To yield one-half maximal inhibition [ND50] of the biological activity of mIL-1b (50 pg/ml), a concentration of 100 - 150 ng/ml of this antibody is required.|
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|IHC-Fr||Use at an assay dependent dilution. PubMed: 18420712Acetone fixed.|
|IHC-FoFr||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
Sandwich ELISA detecting IL1 beta using ab9722 at a concentration of 0.1 µg/ml.