概述

  • 产品名称
    Anti-IL1 alpha抗体
    参阅全部 IL1 alpha 一抗
  • 描述
    兔多克隆抗体to IL1 alpha
  • 经测试应用
    适用于: WB, ELISA, Neutralising, ICC/IFmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Highly pure (>98%) recombinant mIL-1a (mouse Interleukin-1-alpha).

性能

应用

Our Abpromise guarantee covers the use of ab9724 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.1 - 0.2 µg/ml.
ELISA Use at an assay dependent concentration. To detect mIL-1a by sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5 - 2.0 µg/ml of this antibody is required.
Neutralising Use a concentration of 0.03 - 0.05 µg/ml.
ICC/IF Use a concentration of 1 µg/ml.

靶标

  • 功能
    Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
  • 序列相似性
    Belongs to the IL-1 family.
  • 结构域
    The similarity among the IL-1 precursors suggests that the amino ends of these proteins serve some as yet undefined function.
  • 细胞定位
    Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.
  • Information by UniProt
  • 数据库链接
  • 别名
    • BAF antibody
    • FAF antibody
    • Hematopoietin 1 antibody
    • Hematopoietin-1 antibody
    • IL 1 alpha antibody
    • IL 1A antibody
    • IL-1 alpha antibody
    • Il-1a antibody
    • IL1 ALPHA antibody
    • IL1 antibody
    • IL1A antibody
    • IL1A_HUMAN antibody
    • IL1F1 antibody
    • Interleukin 1 alpha antibody
    • Interleukin-1 alpha antibody
    • Interleukin1 alpha antibody
    • LAF antibody
    • LEM antibody
    • Preinterleukin 1 alpha antibody
    • Pro interleukin 1 alpha antibody
    see all

图片

  • Staining of colchicine injected mouse brain (including the hippocampal fissure) tissue. The primary antibody was incubated at 1.0 ug/ml overnight at 4°C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein reagent. Optimal concentrations and conditions may vary.
  • Staining colchicine injected mouse brain (including the hippocampal fissure) tissue. The primary antibody was incubated at 1.0 ug/ml overnight at 4°C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein reagent. Optimal concentrations and conditions may vary.

文献

This product has been referenced in:
  • Rickard JA  et al. RIPK1 regulates RIPK3-MLKL-driven systemic inflammation and emergency hematopoiesis. Cell 157:1175-88 (2014). Read more (PubMed: 24813849) »
  • Nadeau S  et al. Functional Recovery after Peripheral Nerve Injury is Dependent on the Pro-Inflammatory Cytokines IL-1{beta} and TNF: Implications for Neuropathic Pain. J Neurosci 31:12533-12542 (2011). Read more (PubMed: 21880915) »

See all 4 Publications for this product

客户评价及客户问答

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Leica BOND ER1 (pH 6.0)
Permeabilization
No
Specification
Pancreas
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

提交于 Nov 07 2016

Thank you for your reply. I have attached a screenshot of the documentations requirements for this product according to the Automated Import Reference System (AIRS). Regardless of the country of origin for the product, only a commercial invoice...

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Thank you for contacting us. Since this product (ab9724) is classified as an antibody for in vitro use and has been raised in rabbit, no Zoosanitary Export Certificate is needed to ship it to Canada. If you go to this website: http://www...

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Thank you for your enquiry. Our anti-human IL-1 alpha was raised against mature human IL-1 alpha. Please see below the amino acid sequence below for your records. SAPFSFLSNV KYNFMRIIKY EFILNDALNQ SIIRANDQYL TAAALHNLDE AVKFDMGAYK SSKDDAKITV ILRIS...

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Thank you for your enquiry and for your patience. Concerning ab9724, the antibody has not been tested against other species or proteins. Regarding ab10750, all the information that we have on this antibody is located on the online datasheet. If you hav...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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