Anti-IKZF3抗体(ab88513)
Key features and details
- Mouse polyclonal to IKZF3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-IKZF3抗体
参阅全部 IKZF3 一抗 -
描述
小鼠多克隆抗体to IKZF3 -
宿主
Mouse -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein within Human IKZF3. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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阳性对照
- WB: Jurkat cell lysate, IK2F3 transfected 293T cell lysate IHC-P: Human tonsil tissue
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab88513于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 58 kDa.
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IHC-P |
Use a concentration of 3 µg/ml.
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说明 |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 58 kDa. |
IHC-P
Use a concentration of 3 µg/ml. |
靶标
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功能
Transcription factor that plays an important role in the regulation of lymphocyte differentiation. Plays an essential role in regulation of B-cell differentiation, proliferation and maturation to an effector state. Involved in regulating BCL2 expression and controlling apoptosis in T-cells in an IL2-dependent manner. -
组织特异性
Expressed most strongly in peripheral blood leukocytes, the spleen, and the thymus. -
序列相似性
Belongs to the Ikaros C2H2-type zinc-finger protein family.
Contains 6 C2H2-type zinc fingers. -
翻译后修饰
Phosphorylation on tyrosine residues induced by IL2 is required for dissociation from HRAS and nuclear translocation of IKZF3 in T-cells. Phosphorylation on tyrosine residues induced by IL4 is required for dissociation from Bcl-X(L) in T-cells. -
细胞定位
Nucleus. Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 22806 Human
- Omim: 606221 Human
- SwissProt: Q9UKT9 Human
- Unigene: 444388 Human
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别名
- AIO antibody
- Aiolos antibody
- IKAROS family zinc finger 3 (Aiolos) antibody
see all
图片
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Anti-IKZF3 antibody (ab88513) at 1 µg/ml + Jurkat cell lysate at 50 µg
Predicted band size: 58 kDa
Observed band size: 58 kDa
Additional bands at: 51 kDa. We are unsure as to the identity of these extra bands. -
All lanes : Anti-IKZF3 antibody (ab88513) at 1 µg/ml
Lane 1 : IKZF3 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 58 kDa
Observed band size: 58 kDa -
ab88513, at 3 µg/ml, staining IKZF3 in formalin fixed, paraffin embedded human tonsil tissue by Immunoperoxidase/Immunohistochemistry.
数据表及文件
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Datasheet download
文献 (0)
ab88513 尚未被引用在任何文献中。