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RabMAb

Anti-IKK beta抗体[EPR6043] (ab124957)

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概述

  • 产品名称
    Anti-IKK beta抗体[EPR6043]
    参阅全部 IKK beta 一抗
  • 描述
    兔单克隆抗体[EPR6043] to IKK beta
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, IHC-P, Flow Cyt, ICC/IFmore details
    不适用于: IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human IKK beta aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: O14920
    (Peptide available as ab154148)

  • 阳性对照
    • HeLa and 293T cell lysates; Human cervix carcinoma and Mouse kidney tissues; HeLa cells.
  • 常规说明

    A trial size is available to purchase for this antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab124957 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 85 kDa (predicted molecular weight: 87 kDa).Can be blocked with IKK beta peptide (ab154148).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes)
Flow Cyt Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
ICC/IF 1/50 - 1/100.
  • 应用说明
    Is unsuitable for IP.

    • 靶标

    • 功能
      Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Also phosphorylates NCOA3.
    • 组织特异性
      Highly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis and peripheral blood.
    • 序列相似性
      Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
      Contains 1 protein kinase domain.
    • 翻译后修饰
      Upon cytokine stimulation, phosphorylated on Ser-177 and Ser-181 by MEKK1 and/or MAP3K14/NIK; which enhances activity. Once activated, autophosphorylates on the C-terminal serine cluster; which decreases activity and prevents prolonged activation of the inflammatory response.
      Acetylation of Thr-180 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the I-kappa-B pathway.
      Ubiquitinated. Monoubiquitination involves TRIM21 that leads to inhibition of Tax-induced NF-kappa-B signaling. According to PubMed:19675099, 'Ser-163' does not serve as a monoubiquitination site. According to PubMed:16267042, ubiquitination on 'Ser-163' modulates phosphorylation on C-terminal serine residues. Monoubiquitination by TRIM21 is dirupted by Yersinia yopJ.
    • 细胞定位
      Cytoplasm. Membrane raft. Colocalized with DPP4 in membrane rafts.
    • Information by UniProt
    • 数据库链接
      see all
    • 别名
      • I kappa B kinase 2 antibody
      • I kappa B kinase beta antibody
      • I-kappa-B kinase 2 antibody
      • I-kappa-B-kinase beta antibody
      • IkBKB antibody
      • IKK beta antibody
      • IKK-B antibody
      • IKK-beta antibody
      • IKK2 antibody
      • IKKB antibody
      • IKKB_HUMAN antibody
      • IMD15 antibody
      • Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase beta antibody
      • Inhibitor of nuclear factor kappa-B kinase subunit beta antibody
      • NFKBIKB antibody
      • Nuclear factor NF-kappa-B inhibitor kinase beta antibody
      see all

    图片

    • Western blot - Anti-IKK beta antibody [EPR6043] (ab124957)
      Western blot - Anti-IKK beta antibody [EPR6043] (ab124957)

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: IKK beta knockout HAP1 cell lysate (20 µg)
      Lanes 1 and 2: Merged signal (red and green). Green - ab124957 observed at 90 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab124957 was shown to specifically react with IKK beta when IKK beta knockout samples were used. Wild-type and IKK beta knockout samples were subjected to SDS-PAGE. ab124957 and ab8245 (loading control to GAPDH) were diluted 1/500 and 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 h at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKK beta antibody [EPR6043] (ab124957)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKK beta antibody [EPR6043] (ab124957)
      ab124957, at 1/100 dilution staining IKK beta in paraffin-embedded Mouse kidney tissue, by Immunohistochemistry.
    • Western blot - Anti-IKK beta antibody [EPR6043] (ab124957)
      Western blot - Anti-IKK beta antibody [EPR6043] (ab124957)
      All lanes : Anti-IKK beta antibody [EPR6043] (ab124957) at 1/1000 dilution

      Lane 1 : HeLa cell lysates
      Lane 2 : 293T cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

      Predicted band size: 87 kDa

    • Flow Cytometry - Anti-IKK beta antibody [EPR6043] (ab124957)
      Flow Cytometry - Anti-IKK beta antibody [EPR6043] (ab124957)

      Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling IKK beta with unpurified ab124957 at 1/20 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKK beta antibody [EPR6043] (ab124957)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKK beta antibody [EPR6043] (ab124957)
      ab124957, at 1/100 dilution staining IKK beta in paraffin-embedded Human cervix carcinoma tissue, by Immunohistochemistry.
    • Immunocytochemistry/ Immunofluorescence - Anti-IKK beta antibody [EPR6043] (ab124957)
      Immunocytochemistry/ Immunofluorescence - Anti-IKK beta antibody [EPR6043] (ab124957)
      ab124957, at 1/50 dilution, staining IKK beta in HeLa cells by Immunofluorescence.
    • Other - Anti-IKK beta antibody [EPR6043] (ab124957)
      Other - Anti-IKK beta antibody [EPR6043] (ab124957)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    文献

    This product has been referenced in:
    • Li J  et al. Emodin attenuates titanium particle-induced osteolysis and RANKL-mediated osteoclastogenesis through the suppression of IKK phosphorylation. Mol Immunol 96:8-18 (2018). Read more (PubMed: 29455094) »
    • Yu C  et al. Xianyu decoction attenuates the inflammatory response of human lung bronchial epithelial cell. Biomed Pharmacother 102:1092-1098 (2018). WB . Read more (PubMed: 29710526) »

    See all 4 Publications for this product

    发表研究结果有使用 ab124957？请让我们知道，以便我们可以引用本数据表中的参考文章。

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