IKK beta抗体[EPR6043]|Abcam中国|Anti-IKK beta抗体[EPR6043]

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概述

产品名称

Anti-IKK beta抗体[EPR6043]
参阅全部 IKK beta 一抗
描述

兔单克隆抗体[EPR6043] to IKK beta
经测试应用

适用于: WB, IHC-P, Flow Cyt, ICC/IFmore details
不适用于: IP
种属反应性

与反应: Mouse, Rat, Human
免疫原

Synthetic peptide, corresponding to residues at the N terminal of Human IKK beta.
阳性对照
- HeLa and 293T cell lysates; Human cervix carcinoma and Mouse kidney tissues; HeLa cells.
常规说明

A trial size is available to purchase for this antibody.
Produced using Abcam's RabMAb^® technology. RabMAb^® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.
This product is a recombinant rabbit monoclonal antibody.

性能

形式

Liquid
存放说明

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
解离常数（K_D）

K_D = 1.28 x 10 ^-10 M Learn more about K_D
存储溶液

PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
纯度

Tissue culture supernatant
克隆

单克隆
克隆编号

EPR6043
同种型

IgG
研究领域
- Cell Biology
- Apoptosis
- Intracellular
- NFkB
- IKK
- Cancer
- Cell Death
- Apoptosis
- Apoptotic Markers
- NFkB
- IKK

应用

Our Abpromise guarantee covers the use of ab124957 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用	Ab评论	说明
WB		1/1000 - 1/10000. Detects a band of approximately 85 kDa (predicted molecular weight: 87 kDa).Can be blocked with IKK beta peptide (ab154148).
IHC-P		1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes)
Flow Cyt		Use at an assay dependent concentration.
ICC/IF		1/50 - 1/100.

应用说明

Is unsuitable for IP.

靶标

功能

Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Also phosphorylates NCOA3.
组织特异性

Highly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis and peripheral blood.
序列相似性

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
Contains 1 protein kinase domain.
翻译后修饰

Upon cytokine stimulation, phosphorylated on Ser-177 and Ser-181 by MEKK1 and/or MAP3K14/NIK; which enhances activity. Once activated, autophosphorylates on the C-terminal serine cluster; which decreases activity and prevents prolonged activation of the inflammatory response.
Acetylation of Thr-180 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the I-kappa-B pathway.
Ubiquitinated. Monoubiquitination involves TRIM21 that leads to inhibition of Tax-induced NF-kappa-B signaling. According to PubMed:19675099, 'Ser-163' does not serve as a monoubiquitination site. According to PubMed:16267042, ubiquitination on 'Ser-163' modulates phosphorylation on C-terminal serine residues. Monoubiquitination by TRIM21 is dirupted by Yersinia yopJ.
细胞定位

Cytoplasm. Membrane raft. Colocalized with DPP4 in membrane rafts.
Target information above from: UniProt accession O14920 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 3551 Human
- Entrez Gene: 16150 Mouse
- Entrez Gene: 84351 Rat
- Omim: 603258 Human
- SwissProt: O14920 Human
- SwissProt: O88351 Mouse
- SwissProt: Q9QY78 Rat
- Unigene: 597664 Human
- Unigene: 277886 Mouse
- Unigene: 19222 Rat
see all
别名
- I kappa B kinase 2 antibody
- I kappa B kinase beta antibody
- I-kappa-B kinase 2 antibody
- I-kappa-B-kinase beta antibody
- IkBKB antibody
- IKK beta antibody
- IKK-B antibody
- IKK-beta antibody
- IKK2 antibody
- IKKB antibody
- IKKB_HUMAN antibody
- IMD15 antibody
- Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase beta antibody
- Inhibitor of nuclear factor kappa-B kinase subunit beta antibody
- NFKBIKB antibody
- Nuclear factor NF-kappa-B inhibitor kinase beta antibody
see all

Anti-IKK beta antibody [EPR6043] 图像

Western blot - Anti-IKK beta antibody [EPR6043] (ab124957)

Predicted band size : 87 kDa
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: IKK beta knockout HAP1 cell lysate (20 µg)
Lanes 1 and 2: Merged signal (red and green). Green - ab124957 observed at 90 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab124957 was shown to specifically react with IKK beta when IKK beta knockout samples were used. Wild-type and IKK beta knockout samples were subjected to SDS-PAGE. ab124957 and ab8245 (loading control to GAPDH) were diluted 1/500 and 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 h at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKK beta antibody [EPR6043] (ab124957)

ab124957, at 1/100 dilution staining IKK beta in paraffin-embedded Mouse kidney tissue, by Immunohistochemistry.
Western blot - Anti-IKK beta antibody [EPR6043] (ab124957)

All lanes : Anti-IKK beta antibody [EPR6043] (ab124957) at 1/1000 dilution

Lane 1 : HeLa cell lysates
Lane 2 : 293T cell lysates

Lysates/proteins at 10 µg per lane.

Secondary
HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

Predicted band size : 87 kDa
Flow Cytometry - Anti-IKK beta antibody [EPR6043] (ab124957)

Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling IKK beta with unpurified ab124957 at 1/20 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKK beta antibody [EPR6043] (ab124957)

ab124957, at 1/100 dilution staining IKK beta in paraffin-embedded Human cervix carcinoma tissue, by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - Anti-IKK beta antibody [EPR6043] (ab124957)

ab124957, at 1/50 dilution, staining IKK beta in HeLa cells by Immunofluorescence.
Other-Anti-IKK beta antibody [EPR6043](ab124957)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D

实验方案

Flow cytometry protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Anti-IKK beta antibody [EPR6043] (ab124957)参考文献

This product has been referenced in:

Porteiro B et al. Hepatic p63 regulates steatosis via IKKß/ER stress. Nat Commun 8:15111 (2017). WB ; Mouse . Read more (PubMed: 28480888) »

See 1 Publication for this product

发表研究结果有使用 ab124957？请让我们知道，以便我们可以引用本数据表中的参考文章。

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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Anti-IKK beta抗体[EPR6043] (ab124957)

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