使用敲除细胞株进行验证RabMAb

Anti-IKK beta抗体[EPR6043] (ab124957)

概述

  • 产品名称Anti-IKK beta抗体[EPR6043]
    参阅全部 IKK beta 一抗
  • 描述
    兔单克隆抗体[EPR6043] to IKK beta
  • 经测试应用适用于: WB, IHC-P, Flow Cyt, ICC/IFmore details
    不适用于: IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide, corresponding to residues at the N terminal of Human IKK beta.

  • 阳性对照
    • HeLa and 293T cell lysates; Human cervix carcinoma and Mouse kidney tissues; HeLa cells.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

性能

应用

Our Abpromise guarantee covers the use of ab124957 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 85 kDa (predicted molecular weight: 87 kDa).Can be blocked with IKK beta peptide (ab154148).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes)
Flow Cyt Use at an assay dependent concentration.
ICC/IF 1/50 - 1/100.
  • 应用说明Is unsuitable for IP.
  • 靶标

    • 功能Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Also phosphorylates NCOA3.
    • 组织特异性Highly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis and peripheral blood.
    • 序列相似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
      Contains 1 protein kinase domain.
    • 翻译后修饰Upon cytokine stimulation, phosphorylated on Ser-177 and Ser-181 by MEKK1 and/or MAP3K14/NIK; which enhances activity. Once activated, autophosphorylates on the C-terminal serine cluster; which decreases activity and prevents prolonged activation of the inflammatory response.
      Acetylation of Thr-180 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the I-kappa-B pathway.
      Ubiquitinated. Monoubiquitination involves TRIM21 that leads to inhibition of Tax-induced NF-kappa-B signaling. According to PubMed:19675099, 'Ser-163' does not serve as a monoubiquitination site. According to PubMed:16267042, ubiquitination on 'Ser-163' modulates phosphorylation on C-terminal serine residues. Monoubiquitination by TRIM21 is dirupted by Yersinia yopJ.
    • 细胞定位Cytoplasm. Membrane raft. Colocalized with DPP4 in membrane rafts.
    • Information by UniProt
    • 数据库链接
    • 别名
      • I kappa B kinase 2 antibody
      • I kappa B kinase beta antibody
      • I-kappa-B kinase 2 antibody
      • I-kappa-B-kinase beta antibody
      • IkBKB antibody
      • IKK beta antibody
      • IKK-B antibody
      • IKK-beta antibody
      • IKK2 antibody
      • IKKB antibody
      • IKKB_HUMAN antibody
      • IMD15 antibody
      • Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase beta antibody
      • Inhibitor of nuclear factor kappa-B kinase subunit beta antibody
      • NFKBIKB antibody
      • Nuclear factor NF-kappa-B inhibitor kinase beta antibody
      see all

    Anti-IKK beta antibody [EPR6043] 图像



    • Predicted band size : 87 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: IKK beta knockout HAP1 cell lysate (20 µg)
      Lanes 1 and 2: Merged signal (red and green). Green - ab124957 observed at 90 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab124957 was shown to specifically react with IKK beta when IKK beta knockout samples were used. Wild-type and IKK beta knockout samples were subjected to SDS-PAGE. ab124957 and ab8245 (loading control to GAPDH) were diluted 1/500 and 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) ab216776 secondary antibodies at 1/10,000 dilution for 1 h at room temperature before imaging.

    • ab124957, at 1/100 dilution staining IKK beta in paraffin-embedded Mouse kidney tissue, by Immunohistochemistry.
    • All lanes : Anti-IKK beta antibody [EPR6043] (ab124957) at 1/1000 dilution

      Lane 1 : HeLa cell lysates
      Lane 2 : 293T cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

      Predicted band size : 87 kDa
    • Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling IKK beta with unpurified ab124957 at 1/20 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

    • ab124957, at 1/100 dilution staining IKK beta in paraffin-embedded Human cervix carcinoma tissue, by Immunohistochemistry.
    • ab124957, at 1/50 dilution, staining IKK beta in HeLa cells by Immunofluorescence.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Anti-IKK beta antibody [EPR6043] (ab124957)参考文献

    ab124957 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab124957.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"