Anti-IKK beta抗体[EPR6043] (ab124957)
- 数据表
- 具体的参考文献 (2)
- 实验方案
概述
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产品名称Anti-IKK beta抗体[EPR6043]
参阅全部 IKK beta 一抗 -
描述兔单克隆抗体[EPR6043] to IKK beta
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宿主Rabbit
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经测试应用适用于: WB, IHC-P, Flow Cyt, ICC/IFmore details
不适用于: IP -
种属反应性与反应: Mouse, Rat, Human
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免疫原
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阳性对照
- HeLa and 293T cell lysates; Human cervix carcinoma and Mouse kidney tissues; HeLa cells.
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常规说明
A trial size is available to purchase for this antibody.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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解离常数（KD）KD = 1.28 x 10 -10 M Learn more about KD
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存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
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纯度Tissue culture supernatant
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克隆单克隆
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克隆编号EPR6043
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同种型IgG
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研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Positive Controls
应用
Our Abpromise guarantee covers the use of ab124957 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 85 kDa (predicted molecular weight: 87 kDa).Can be blocked with IKK beta peptide (ab154148). | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. (Heat to 98°C, allow to cool for 10-20 minutes) | |
| Flow Cyt | Use at an assay dependent concentration. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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| ICC/IF | 1/50 - 1/100. |
靶标
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功能Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Also phosphorylates NCOA3.
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组织特异性Highly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis and peripheral blood.
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序列相似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
Contains 1 protein kinase domain. -
翻译后修饰Upon cytokine stimulation, phosphorylated on Ser-177 and Ser-181 by MEKK1 and/or MAP3K14/NIK; which enhances activity. Once activated, autophosphorylates on the C-terminal serine cluster; which decreases activity and prevents prolonged activation of the inflammatory response.
Acetylation of Thr-180 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the I-kappa-B pathway.
Ubiquitinated. Monoubiquitination involves TRIM21 that leads to inhibition of Tax-induced NF-kappa-B signaling. According to PubMed:19675099, 'Ser-163' does not serve as a monoubiquitination site. According to PubMed:16267042, ubiquitination on 'Ser-163' modulates phosphorylation on C-terminal serine residues. Monoubiquitination by TRIM21 is dirupted by Yersinia yopJ. -
细胞定位Cytoplasm. Membrane raft. Colocalized with DPP4 in membrane rafts.
- Information by UniProt
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数据库链接
- Entrez Gene: 3551 Human
- Entrez Gene: 16150 Mouse
- Entrez Gene: 84351 Rat
- Omim: 603258 Human
- SwissProt: O14920 Human
- SwissProt: O88351 Mouse
- SwissProt: Q9QY78 Rat
- Unigene: 597664 Human
see all -
别名
- I kappa B kinase 2 antibody
- I kappa B kinase beta antibody
- I-kappa-B kinase 2 antibody
see all
图片
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![Western blot - Anti-IKK beta antibody [EPR6043] (ab124957)](http://www.abcam.cn/ps/products/124/ab124957/Images/ab124957-256177-ab124957KOWB.jpg)
Western blot - Anti-IKK beta antibody [EPR6043] (ab124957)Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: IKK beta knockout HAP1 cell lysate (20 µg)
Lanes 1 and 2: Merged signal (red and green). Green - ab124957 observed at 90 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab124957 was shown to specifically react with IKK beta when IKK beta knockout samples were used. Wild-type and IKK beta knockout samples were subjected to SDS-PAGE. ab124957 and ab8245 (loading control to GAPDH) were diluted 1/500 and 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 h at room temperature before imaging. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKK beta antibody [EPR6043] (ab124957)](http://www.abcam.cn/ps/products/124/ab124957/Images/IKK-beta-Primary-antibodies-ab124957-3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKK beta antibody [EPR6043] (ab124957)ab124957, at 1/100 dilution staining IKK beta in paraffin-embedded Mouse kidney tissue, by Immunohistochemistry. -
![Western blot - Anti-IKK beta antibody [EPR6043] (ab124957)](http://www.abcam.cn/ps/products/124/ab124957/Images/IKK-beta-Primary-antibodies-ab124957-1.jpg)
Western blot - Anti-IKK beta antibody [EPR6043] (ab124957)All lanes : Anti-IKK beta antibody [EPR6043] (ab124957) at 1/1000 dilution
Lane 1 : HeLa cell lysates
Lane 2 : 293T cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution
Predicted band size: 87 kDa -
![Flow Cytometry - Anti-IKK beta antibody [EPR6043] (ab124957)](http://www.abcam.cn/ps/products/124/ab124957/Images/ab124957-270663-ab124957FC1.jpg)
Flow Cytometry - Anti-IKK beta antibody [EPR6043] (ab124957)Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling IKK beta with unpurified ab124957 at 1/20 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKK beta antibody [EPR6043] (ab124957)](http://www.abcam.cn/ps/products/124/ab124957/Images/IKK-beta-Primary-antibodies-ab124957-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKK beta antibody [EPR6043] (ab124957)ab124957, at 1/100 dilution staining IKK beta in paraffin-embedded Human cervix carcinoma tissue, by Immunohistochemistry. -
![Immunocytochemistry/ Immunofluorescence - Anti-IKK beta antibody [EPR6043] (ab124957)](http://www.abcam.cn/ps/products/124/ab124957/Images/IKK-beta-Primary-antibodies-ab124957-4.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-IKK beta antibody [EPR6043] (ab124957)ab124957, at 1/50 dilution, staining IKK beta in HeLa cells by Immunofluorescence. -
](http://www.abcam.cn/ps/products/124/ab124957/Images/IKK-beta-Primary-antibodies-ab124957-5.jpg)
Other-Anti-IKK beta antibody [EPR6043](ab124957)
实验方案
文献
This product has been referenced in:
- Porteiro B et al. Hepatic p63 regulates steatosis via IKKß/ER stress. Nat Commun 8:15111 (2017). WB ; Mouse . Read more (PubMed: 28480888) »
- Zhan J et al. Upregulation of neuronal zinc finger protein A20 expression is required for electroacupuncture to attenuate the cerebral inflammatory injury mediated by the nuclear factor-kB signaling pathway in cerebral ischemia/reperfusion rats. J Neuroinflammation 13:258 (2016). Read more (PubMed: 27716383) »
客户评价及客户问答
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