概述

  • 产品名称Anti-IKK beta抗体
    参阅全部 IKK beta 一抗
  • 描述
    兔多克隆抗体to IKK beta
  • 特异性Control peptide (ab 7612) will compete only with the specific reaction of antiserum with the IKKb subunit.
  • 经测试应用适用于: WB, IP, ELISA, Dot blotmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to IKK beta (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • 阳性对照
    • HeLa cell lysate.

性能

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应用

Our Abpromise guarantee covers the use of ab7611 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
IP
ELISA
Dot blot
  • 应用说明Dot: Use at an assay dependant dilution.
    ELISA: Use at an assay dependant dilution.
    IP: Use at an assay dependant dilution.
    WB: 1/1000. Detects a band of approximately 87 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Also phosphorylates NCOA3.
    • 组织特异性Highly expressed in heart, placenta, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis and peripheral blood.
    • 序列相似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
      Contains 1 protein kinase domain.
    • 翻译后修饰Upon cytokine stimulation, phosphorylated on Ser-177 and Ser-181 by MEKK1 and/or MAP3K14/NIK; which enhances activity. Once activated, autophosphorylates on the C-terminal serine cluster; which decreases activity and prevents prolonged activation of the inflammatory response.
      Acetylation of Thr-180 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the I-kappa-B pathway.
      Ubiquitinated. Monoubiquitination involves TRIM21 that leads to inhibition of Tax-induced NF-kappa-B signaling. According to PubMed:19675099, 'Ser-163' does not serve as a monoubiquitination site. According to PubMed:16267042, ubiquitination on 'Ser-163' modulates phosphorylation on C-terminal serine residues. Monoubiquitination by TRIM21 is dirupted by Yersinia yopJ.
    • 细胞定位Cytoplasm. Membrane raft. Colocalized with DPP4 in membrane rafts.
    • Information by UniProt
    • 数据库链接
    • 别名
      • I kappa B kinase 2 antibody
      • I kappa B kinase beta antibody
      • I-kappa-B kinase 2 antibody
      • I-kappa-B-kinase beta antibody
      • IkBKB antibody
      • IKK beta antibody
      • IKK-B antibody
      • IKK-beta antibody
      • IKK2 antibody
      • IKKB antibody
      • IKKB_HUMAN antibody
      • IMD15 antibody
      • Inhibitor of kappa light polypeptide gene enhancer in B cells, kinase beta antibody
      • Inhibitor of nuclear factor kappa-B kinase subunit beta antibody
      • NFKBIKB antibody
      • Nuclear factor NF-kappa-B inhibitor kinase beta antibody
      see all

    Anti-IKK beta antibody (ab7611)参考文献

    This product has been referenced in:
    • Tang Y  et al. Inhibition of the IKK/NF-?B pathway by AAV gene transfer improves muscle regeneration in older mdx mice. Gene Ther 17:1476-83 (2010). Read more (PubMed: 20720575) »

    See 1 Publication for this product

    Product Wall

    Thank you for your enquiry. The 80 mg/ml is the total protein concentration (as determined by Refractometry) and is not the antibody concentration. The purity of this product is indeed whole antiserum and as it is unpurified, an exact antibody concentr...

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    I'm sorry to hear that you are having problems with ab7611 in WB. I would recommend removing the milk from your protocol as this may be the source of the non-specific binding and to increase the amount of antibody (trying 1:300-1:500) as the diluti...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"