Anti-IKK alpha (phospho S176)抗体(ab138426)
Key features and details
- Rabbit polyclonal to IKK alpha (phospho S176)
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
概述
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产品名称
Anti-IKK alpha (phospho S176)抗体
参阅全部 IKK alpha 一抗 -
描述
兔多克隆抗体to IKK alpha (phospho S176) -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse
预测可用于: Rat -
免疫原
Synthetic peptide corresponding to Human IKK alpha (internal sequence) (phospho S176).
Database link: O15111 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20ºC. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.88% Sodium chloride
PBS is without Mg2+ and Ca2+ -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
ab138426 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab138426于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
1/500 - 1/1000. Predicted molecular weight: 84 kDa.
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说明 |
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WB
1/500 - 1/1000. Predicted molecular weight: 84 kDa. |
靶标
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功能
Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. Also phosphorylates NCOA3. Phosphorylates 'Ser-10' of histone H3 at NF-kappa-B-regulated promoters during inflammatory responses triggered by cytokines. -
组织特异性
Widely expressed. -
疾病相关
Defects in CHUK are the cause of cocoon syndrome (COCOS) [MIM:613630]; also known as fetal encasement syndrome. COCOS is a lethal syndrome characterized by multiple fetal malformations including defective face and seemingly absent limbs, which are bound to the trunk and encased under the skin. -
序列相似性
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
Contains 1 protein kinase domain. -
翻译后修饰
Phosphorylated by MAP3K14/NIK, AKT and to a lesser extent by MEKK1, and dephosphorylated by PP2A. Autophosphorylated.
Acetylation of Thr-179 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the I-kappa-B signaling pathway. -
细胞定位
Cytoplasm. Nucleus. Shuttles between the cytoplasm and the nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 309361 Rat
- SwissProt: Q60680 Mouse
- Unigene: 3996 Mouse
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别名
- chuk antibody
- CHUK1 antibody
- Conserved Helix Loop Helix Ubiquitous Kinase antibody
see all
图片
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab138426 被引用在 5 文献中.
- Liang ZW et al. Tumor-associated macrophages promote the metastasis and growth of non-small-cell lung cancer cells through NF-?B/PP2Ac-positive feedback loop. Cancer Sci 112:2140-2157 (2021). PubMed: 33609307
- Montes M et al. Determining the prognostic significance of IKKa in prostate cancer. Prostate 80:1188-1202 (2020). PubMed: 33258506
- Qu M et al. Yi-Qi-Ping-Chuan-Fang Reduces TSLP Elevation Caused by LPS + Poly(I:C) via Inhibiting TLR4/MYD88/NF-?B Signaling Pathway. Evid Based Complement Alternat Med 2017:3209407 (2017). PubMed: 29250125
- Wang C et al. HTLV-1 Tax Functions as a Ubiquitin E3 Ligase for Direct IKK Activation via Synthesis of Mixed-Linkage Polyubiquitin Chains. PLoS Pathog 12:e1005584 (2016). PubMed: 27082114
- Tao M et al. Inflammatory stimuli promote growth and invasion of pancreatic cancer cells through NF-?B pathway dependent repression of PP2Ac. Cell Cycle 15:381-93 (2016). PubMed: 26761431