Anti-IKK alpha抗体(ab4111)


  • 产品名称Anti-IKK alpha抗体
    参阅全部 IKK alpha 一抗
  • 描述
    兔多克隆抗体to IKK alpha
  • 特异性Recognises human IKK alpha and does not cross react with IKK beta or IKK gamma.
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to amino acids 716-734 of human IKK alpha (1,2), which differs from correspondingmurine sequence by four amino acids.

  • 阳性对照
    • Whole cell lysate from HeLa or Jurkat cells.




Our Abpromise guarantee covers the use of ab4111 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
  • 应用说明ICC/IF: Use at a concentration of 1 µg/ml.
    WB: At 1/1000-1/2000 dilution a 85kDa band should be detected.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. Also phosphorylates NCOA3. Phosphorylates 'Ser-10' of histone H3 at NF-kappa-B-regulated promoters during inflammatory responses triggered by cytokines.
    • 组织特异性Widely expressed.
    • 疾病相关Defects in CHUK are the cause of cocoon syndrome (COCOS) [MIM:613630]; also known as fetal encasement syndrome. COCOS is a lethal syndrome characterized by multiple fetal malformations including defective face and seemingly absent limbs, which are bound to the trunk and encased under the skin.
    • 序列相似性Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
      Contains 1 protein kinase domain.
    • 翻译后修饰Phosphorylated by MAP3K14/NIK, AKT and to a lesser extent by MEKK1, and dephosphorylated by PP2A. Autophosphorylated.
      Acetylation of Thr-179 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the I-kappa-B signaling pathway.
    • 细胞定位Cytoplasm. Nucleus. Shuttles between the cytoplasm and the nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • chuk antibody
      • CHUK1 antibody
      • Conserved Helix Loop Helix Ubiquitous Kinase antibody
      • Conserved helix loop ubiquitous kinase antibody
      • Conserved helix-loop-helix ubiquitous kinase antibody
      • I Kappa B Kinase 1 antibody
      • I Kappa B Kinase Alpha antibody
      • I-kappa-B kinase 1 antibody
      • I-kappa-B kinase alpha antibody
      • IkappaB kinase antibody
      • IkB kinase alpha subunit antibody
      • IkBKA antibody
      • IKK 1 antibody
      • IKK A antibody
      • IKK a kinase antibody
      • IKK-A antibody
      • IKK-alpha antibody
      • IKK1 antibody
      • IKKA antibody
      • IKKA_HUMAN antibody
      • Inhibitor Of Kappa Light Polypeptide Gene Enhancer In B Cells antibody
      • Inhibitor Of Nuclear Factor Kappa B Kinase Alpha Subunit antibody
      • Inhibitor of nuclear factor kappa-B kinase subunit alpha antibody
      • NFKBIKA antibody
      • Nuclear Factor Kappa B Inhibitor Kinase Alpha antibody
      • Nuclear factor NF kappa B inhibitor kinase alpha antibody
      • Nuclear factor NF-kappa-B inhibitor kinase alpha antibody
      • Nuclear factor NFkappaB inhibitor kinase alpha antibody
      • Nuclear Factor Of Kappa Light Chain Gene Enhancer In B Cells Inhibitor antibody
      • TCF-16 antibody
      • TCF16 antibody
      • Transcription factor 16 antibody
      see all

    Anti-IKK alpha antibody 图像

    • Anti-IKK alpha antibody (ab4111) at 1/1000 dilution + HeLa whole cell lysate

      Observed band size : 85 kDa (why is the actual band size different from the predicted?)
    • ab4111 at 1µg/ml staining IKK alpha in Jurkat cells by ICC/IF

    • Immunofluorescence of IKK alpha in Jurkat cells using ab4111 at 10 ug/ml.

    Anti-IKK alpha antibody (ab4111)参考文献

    ab4111 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HeLa cell extract)
    Loading amount 50000 cells
    Specification HeLa cell extract
    Gel Running Conditions Reduced Denaturing
    Blocking step Pierce TBS Super Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C

    Dr. Mahesh Mathrubutham

    Verified customer

    提交于 Nov 21 2007

    All the information we have for our products is on the datasheets. As well as reviewing the applications section to determine whether ICC/IHC has been tested, I also suggest that you review the cross reactivity section. For example, this antibody -- ab...

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