RabMAb

Anti-IKB beta抗体[EPR5037] (ab109509)

概述

  • 产品名称Anti-IKB beta抗体[EPR5037]
    参阅全部 IKB beta 一抗
  • 描述
    兔单克隆抗体[EPR5037] to IKB beta
  • 经测试应用适用于: WB, IP, IHC-P, ICCmore details
    不适用于: Flow Cyt
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to residues on the C-terminus in Human IKB beta.

  • 阳性对照
    • Jurkat, HeLa, THP-1, and MCF7 cell lysates, Human kidney tissue
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab109509 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 37 kDa).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC 1/100 - 1/250.
  • 应用说明Is unsuitable for Flow Cyt.
  • 靶标

    • 功能Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. However, the unphosphorylated form resynthesized after cell stimulation is able to bind NF-kappa-B allowing its transport to the nucleus and protecting it to further NFKBIA-dependent inactivation. Association with inhibitor kappa B-interacting NKIRAS1 and NKIRAS2 prevent its phosphorylation rendering it more resistant to degradation, explaining its slower degradation.
    • 组织特异性Expressed in all tissues examined.
    • 序列相似性Belongs to the NF-kappa-B inhibitor family.
      Contains 6 ANK repeats.
    • 翻译后修饰Phosphorylated by RPS6KA1; followed by degradation. Interaction with NKIRAS1 and NKIRAS2 probably prevents phosphorylation.
    • 细胞定位Cytoplasm. Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • I kappa B beta antibody
      • I-kappa-B-beta antibody
      • IkappaBbeta antibody
      • IKB beta antibody
      • IkB-B antibody
      • IkB-beta antibody
      • IKBB antibody
      • IKBB_HUMAN antibody
      • IkBbeta antibody
      • NF kappa BIB antibody
      • NF-kappa-B inhibitor beta antibody
      • NF-kappa-BIB antibody
      • Nfkbib antibody
      • Thyroid receptor interacting protein 9 antibody
      • Thyroid receptor-interacting protein 9 antibody
      • TR interacting protein 9 antibody
      • TR-interacting protein 9 antibody
      • TRIP-9 antibody
      • TRIP9 antibody
      see all

    Anti-IKB beta antibody [EPR5037] 图像

    • All lanes : Anti-IKB beta antibody [EPR5037] (ab109509) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : THP-1 cell lysate
      Lane 4 : MCF7 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 37 kDa
      Observed band size : 48 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue using ab109509 at a dilution of 1/100.

    Anti-IKB beta antibody [EPR5037] (ab109509)参考文献

    ab109509 has not yet been referenced specifically in any publications.

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