概述

  • 产品名称Anti-IKB beta抗体
    参阅全部 IKB beta 一抗
  • 描述
    兔多克隆抗体to IKB beta
  • 经测试应用适用于: ICC/IF, WB, IP, ELISA, Dot blotmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human IKB beta (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • 阳性对照
    • A control peptide is available, ab7548.

性能

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应用

Our Abpromise guarantee covers the use of ab7547 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100.
WB 1/500 - 1/3000. Detects a band of approximately 45 kDa (predicted molecular weight: 38 kDa).
IP Use at an assay dependent concentration.
ELISA 1/5000 - 1/25000.
Dot blot Use at an assay dependent concentration.

靶标

  • 功能Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. However, the unphosphorylated form resynthesized after cell stimulation is able to bind NF-kappa-B allowing its transport to the nucleus and protecting it to further NFKBIA-dependent inactivation. Association with inhibitor kappa B-interacting NKIRAS1 and NKIRAS2 prevent its phosphorylation rendering it more resistant to degradation, explaining its slower degradation.
  • 组织特异性Expressed in all tissues examined.
  • 序列相似性Belongs to the NF-kappa-B inhibitor family.
    Contains 6 ANK repeats.
  • 翻译后修饰Phosphorylated by RPS6KA1; followed by degradation. Interaction with NKIRAS1 and NKIRAS2 probably prevents phosphorylation.
  • 细胞定位Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • I kappa B beta antibody
    • I-kappa-B-beta antibody
    • IkappaBbeta antibody
    • IKB beta antibody
    • IkB-B antibody
    • IkB-beta antibody
    • IKBB antibody
    • IKBB_HUMAN antibody
    • IkBbeta antibody
    • NF kappa BIB antibody
    • NF-kappa-B inhibitor beta antibody
    • NF-kappa-BIB antibody
    • Nfkbib antibody
    • Thyroid receptor interacting protein 9 antibody
    • Thyroid receptor-interacting protein 9 antibody
    • TR interacting protein 9 antibody
    • TR-interacting protein 9 antibody
    • TRIP-9 antibody
    • TRIP9 antibody
    see all

Anti-IKB beta antibody 图像

  • Detection of IkB beta by Immunoprecipitation in whole cell lysate from Huh-7 cells, using ab7547 at 4 µg/mg lysate for IP and ab1162 at a 1/1500 dilution for subsequent Western blot detection.
    Cells expressed recombinant IkB beta tagged with DDDDK. Protein A was used for the Immunoprecipitation step. Primary antibody incubated with matrix for 4 hours at 4°C.

    See Abreview

  • ICC/IF image of ab7547 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7547, 1/100 dilution) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-IKB beta antibody (ab7547)参考文献

This product has been referenced in:
  • Stewart ME & Roy P Role of cellular caspases, nuclear factor-kappa B and interferon regulatory factors in Bluetongue virus infection and cell fate. Virol J 7:362 (2010). WB ; Human . Read more (PubMed: 21134281) »
  • De Barros S  et al. Inhibition of human preadipocyte proteasomal activity by HIV protease inhibitors or specific inhibitor lactacystin leads to a defect in adipogenesis, which involves matrix metalloproteinase-9. J Pharmacol Exp Ther 320:291-9 (2007). Read more (PubMed: 17038510) »

See all 2 Publications for this product

Product Wall

Merci de nous avoir contactés.

Suite à notre conversation téléphonique d'hier, j’ai contacté notre laboratoire a propos de la concentration d’ab7547. Comme l’anticorps est un antisér...

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Thank you for contacting us. Because we carry over 70,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guar...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (huh-7)
Total protein in input 1e+006 cells
Specification huh-7
Treatment cells expressing recombitant IkB beta tagged with DDDDK
Immuno-precipitation step Protein A
Username

Abcam user community

Verified customer

提交于 Sep 15 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (huh-7)
Loading amount 15000 cells
Specification huh-7
Treatment cells expressing recombitant human IkB-beta
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Nov 12 2008

Thank you for your enquiry and your patience. Anti- IKBbeta antibody may react non-specifically with other proteins. We don't know if you have ordered the control peptide (Ab7548) which will compete only with the specific reaction of antiserum with IKB...

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Thank you for your enquiry. We do not have any information on optimal Western conditions for this antibody, only that it be used at a dilution of 1/1000. I do not know if it is necessary to incubate the blot with primary overnight. Our Western blot pro...

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I'm afraid that we do not have a blot image of this antibody that we could forward to you. However, please be assured that if you decide to purchase this antibody and it does not work as we specify on the datasheet, we will send you a replacement vial ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"