概述

  • 产品名称Anti-IKB beta抗体
    参阅全部 IKB beta 一抗
  • 描述
    小鼠多克隆抗体to IKB beta
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Rat
    预测可用于: Mouse
  • 免疫原

    Recombinant fusion protein (tagged): EKLYAAGAGV LVTERGGHTA LHLACRVRAH TCAYVLLQPR PSHPRDASDT YLTQSQDHTP DTSHAPVATD PQPNPGNEEE LRDEDWRLQL EAENYDGHTP , corresponding to amino acids 111-210 of Rat IKK beta

  • 常规说明


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

性能

应用

Our Abpromise guarantee covers the use of ab52775 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000.

靶标

  • 功能Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. However, the unphosphorylated form resynthesized after cell stimulation is able to bind NF-kappa-B allowing its transport to the nucleus and protecting it to further NFKBIA-dependent inactivation. Association with inhibitor kappa B-interacting NKIRAS1 and NKIRAS2 prevent its phosphorylation rendering it more resistant to degradation, explaining its slower degradation.
  • 组织特异性Expressed in all tissues examined.
  • 序列相似性Belongs to the NF-kappa-B inhibitor family.
    Contains 6 ANK repeats.
  • 翻译后修饰Phosphorylated by RPS6KA1; followed by degradation. Interaction with NKIRAS1 and NKIRAS2 probably prevents phosphorylation.
  • 细胞定位Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • I kappa B beta antibody
    • I-kappa-B-beta antibody
    • IkappaBbeta antibody
    • IKB beta antibody
    • IkB-B antibody
    • IkB-beta antibody
    • IKBB antibody
    • IKBB_HUMAN antibody
    • IkBbeta antibody
    • NF kappa BIB antibody
    • NF-kappa-B inhibitor beta antibody
    • NF-kappa-BIB antibody
    • Nfkbib antibody
    • Thyroid receptor interacting protein 9 antibody
    • Thyroid receptor-interacting protein 9 antibody
    • TR interacting protein 9 antibody
    • TR-interacting protein 9 antibody
    • TRIP-9 antibody
    • TRIP9 antibody
    see all

Anti-IKB beta antibody 图像

  • All lanes : Anti-IKB beta antibody (ab52775) at 1/1000 dilution

    Lane 1 : a total protein extract from E coli with 50ng to 100 ng of a tagged fusion protein of an irrelevant antigen.
    Lane 2 : a total protein extract from E coli with 50ng to 500ng of the antigen (tagged antigen fusion protein)

    Lysates/proteins at 20 µg per lane.

    Secondary
    Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase at 1/5000 dilution

    Observed band size : 38 kDa (why is the actual band size different from the predicted?)

Anti-IKB beta antibody (ab52775)参考文献

ab52775 has not yet been referenced specifically in any publications.

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