Anti-IKB alpha (phospho Y42)抗体(ab24783)
Key features and details
- Rabbit polyclonal to IKB alpha (phospho Y42)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-IKB alpha (phospho Y42)抗体
参阅全部 IKB alpha 一抗 -
描述
兔多克隆抗体to IKB alpha (phospho Y42) -
宿主
Rabbit -
特异性
There may be some homology to unrelated proteins with a conserved tyrosine phosphorylation motif. It is recommended that this antibody be used after immunoprecipitation with an IKB alpha specific antibody. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human IKB alpha (phospho Y42) conjugated to keyhole limpet haemocyanin.
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常规说明
We do not have concentration information for this antibody. It is optimized to work at the dilutions stated on the datasheet.Useful in detection of phosphorylated IKB alpha (Tyr-42).
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Cross absorbed to phospho-tyrosine coupled to agarose then affinity purified using phospho-IKB alpha (Tyr-42) peptide, without carrier. -
Primary antibody说明
Useful in detection of phosphorylated IKB alpha (Tyr-42). -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab24783于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000. Detects a band of approximately 38 kDa (predicted molecular weight: 36 kDa).
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说明 |
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WB
1/1000. Detects a band of approximately 38 kDa (predicted molecular weight: 36 kDa). |
靶标
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功能
Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription. -
疾病相关
Ectodermal dysplasia, anhidrotic, with T-cell immunodeficiency autosomal dominant -
序列相似性
Belongs to the NF-kappa-B inhibitor family.
Contains 5 ANK repeats. -
翻译后修饰
Phosphorylated; disables inhibition of NF-kappa-B DNA-binding activity. Phosphorylation at positions 32 and 36 is prerequisite to recognition by UBE2D3 leading to polyubiquitination and subsequent degradation.
Sumoylated; sumoylation requires the presence of the nuclear import signal. Sumoylation blocks ubiquitination and proteasome-mediated degradation of the protein thereby increasing the protein stability.
Monoubiquitinated at Lys-21 and/or Lys-22 by UBE2D3. Ubiquitin chain elongation is then performed by CDC34 in cooperation with the SCF(FBXW11) E3 ligase complex, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. The resulting polyubiquitination leads to protein degradation. Also ubiquitinated by SCF(BTRC) following stimulus-dependent phosphorylation at Ser-32 and Ser-36.
Deubiquitinated by porcine reproductive and respiratory syndrome virus Nsp2 protein, which thereby interferes with NFKBIA degradation and impairs subsequent NF-kappa-B activation. -
细胞定位
Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export. - Information by UniProt
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数据库链接
- Entrez Gene: 4792 Human
- Omim: 164008 Human
- SwissProt: P25963 Human
- Unigene: 81328 Human
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别名
- I kappa B alpha antibody
- I-kappa-B-alpha antibody
- IkappaBalpha antibody
see all
图片
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Western blot analysis of A431 cells treated with pervanadate (1 mM) for 30 minutes (30 ug/lane). Blots were probed with anti-IkappaBalpha (lane 1), anti-phospho-IkappaBalpha?(Tyr-42) (ab24783; lanes 2-5), or anti-phospho-IkappaBalpha (Tyr-305) (ab24784; lanes 6-9). Both anti-phospho-IkappaBalpha (Tyr-42) and anti-phospho-IkappaBalpha (Tyr-305) were used in the presence of no blocking peptide (lane 2 & 6), phospho-IkappaBalpha (Tyr-42) peptide (lane 3 & 8), phospho-IkappaBalpha (Tyr-305) peptide (lane 4 & 7), or BSA conjugated to phospho-tyrosine (lane 5 & 9). Peptides and BSA-pTyr were used at 1 ug/ml.
数据表及文件
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SDS download
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Datasheet download
文献 (19)
ab24783 被引用在 19 文献中.
- Shi CX et al. Sevoflurane attenuates brain damage through inhibiting autophagy and apoptosis in cerebral ischemia-reperfusion rats. Mol Med Rep 21:123-130 (2020). PubMed: 31746402
- Luo Y et al. Taurine attenuates liver autophagy and injury of offspring in gestational diabetic mellitus rats. Life Sci 257:117889 (2020). PubMed: 32502541
- Lv H et al. Swainsonine protects H9c2 cells against lipopolysaccharide-induced apoptosis and inflammatory injury via down-regulating miR-429. Cell Cycle 19:207-217 (2020). PubMed: 31876239
- Meng Y et al. Knockdown of exosome-mediated lnc-PVT1 alleviates lipopolysaccharide-induced osteoarthritis progression by mediating the HMGB1/TLR4/NF-?B pathway via miR-93-5p. Mol Med Rep 22:5313-5325 (2020). PubMed: 33174011
- Li T et al. Silencing cyclophilin A improves insulin secretion, reduces cell apoptosis, and alleviates inflammation as well as oxidant stress in high glucose-induced pancreatic ß-cells via MAPK/NF-kb signaling pathway. Bioengineered 11:1047-1057 (2020). PubMed: 32970961