Anti-IKB alpha (phospho Y42)抗体(ab24783)

概述

• 产品名称
  Anti-IKB alpha (phospho Y42)抗体
  参阅全部 IKB alpha 一抗
• 描述
  兔多克隆抗体to IKB alpha (phospho Y42)
• 特异性
  There may be some homology to unrelated proteins with a conserved tyrosine phosphorylation motif. It is recommended that this antibody be used after immunoprecipitation with an IKB alpha specific antibody.
• 经测试应用
  适用于: WB, IP, ELISAmore details
• 种属反应性
  与反应: Mouse, Human
  
• 免疫原

  Synthetic peptide corresponding to amino acid residues around tyrosine 42 of human IKB alpha, conjugated to KLH

• 阳性对照
  • Pervanadate treated A431 and Jurkat cells.
• 常规说明
  We do not have concentration information for this antibody. It is optimized to work at the dilutions stated on the datasheet.

  
  

  Useful in detection of phosphorylated IKB alpha (Tyr-42).

性能

• 形式
  Liquid
• 存放说明
  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• 存储溶液
  Preservative: 0.05% Sodium Azide
  Constituents: 50% Glycerol, PBS, 1mg/ml BSA
• Concentration information loading...
• 纯度
  Immunogen affinity purified
• 纯化说明
  Cross absorbed to phospho-tyrosine coupled to agarose then affinity purified using phospho-IKB alpha (Tyr-42) peptide, without carrier.
• Primary antibody说明
  Useful in detection of phosphorylated IKB alpha (Tyr-42).
• 克隆
  多克隆
• 同种型
  IgG
• 研究领域
  • Signal Transduction
  • Signaling Pathway
  • Nuclear Signaling
  • NFkB Pathway

  • Epigenetics and Nuclear Signaling
  • Nuclear Signaling Pathways
  • NFkB pathway

  • Cancer
  • Signal transduction
  • Nuclear signaling
  • NFkB pathway

  • Immunology
  • Innate Immunity
  • TLR Signaling

相关产品

• Compatible Secondaries
  • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

应用

靶标

• 功能
  Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.
• 疾病相关
  Ectodermal dysplasia, anhidrotic, with T-cell immunodeficiency autosomal dominant
• 序列相似性
  Belongs to the NF-kappa-B inhibitor family.
  Contains 5 ANK repeats.
• 翻译后修饰
  Phosphorylated; disables inhibition of NF-kappa-B DNA-binding activity. Phosphorylation at positions 32 and 36 is prerequisite to recognition by UBE2D3 leading to polyubiquitination and subsequent degradation.
  Sumoylated; sumoylation requires the presence of the nuclear import signal. Sumoylation blocks ubiquitination and proteasome-mediated degradation of the protein thereby increasing the protein stability.
  Monoubiquitinated at Lys-21 and/or Lys-22 by UBE2D3. Ubiquitin chain elongation is then performed by CDC34 in cooperation with the SCF(FBXW11) E3 ligase complex, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. The resulting polyubiquitination leads to protein degradation. Also ubiquitinated by SCF(BTRC) following stimulus-dependent phosphorylation at Ser-32 and Ser-36.
  Deubiquitinated by porcine reproductive and respiratory syndrome virus Nsp2 protein, which thereby interferes with NFKBIA degradation and impairs subsequent NF-kappa-B activation.
• 细胞定位
  Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export.

• Target information above from: UniProt accession P25963 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• 数据库链接
  • Entrez Gene: 4792 Human
  • Entrez Gene: 18035 Mouse
  • Omim: 164008 Human
  • SwissProt: P25963 Human
  • SwissProt: Q9Z1E3 Mouse
  • Unigene: 81328 Human
  • Unigene: 170515 Mouse
• 别名
  • I kappa B alpha antibody
  • I-kappa-B-alpha antibody
  • IkappaBalpha antibody

  • IkB-alpha antibody
  • IKBA antibody
  • IKBA_HUMAN antibody
  • IKBalpha antibody
  • MAD 3 antibody
  • MAD3 antibody
  • Major histocompatibility complex enhancer-binding protein MAD3 antibody
  • NF kappa B inhibitor alpha antibody
  • NF-kappa-B inhibitor alpha antibody
  • NFKBI antibody
  • NFKBIA antibody
  • Nuclear factor of kappa light chain gene enhancer in B cells antibody
  • Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha antibody

  see all

实验方案

• Western blot protocols
• Immunoprecipitation protocols