概述

  • 产品名称Anti-IKB alpha抗体
    参阅全部 IKB alpha 一抗
  • 描述
    兔多克隆抗体to IKB alpha
  • 经测试应用适用于: IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Pig
  • 免疫原

    Recombinant fragment corresponding to Human IKB alpha (internal sequence). a sequence within the center region
    Database link: P25963

  • 阳性对照
    • 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji whole cell lysates; HeLa cells.

性能

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应用

Our Abpromise guarantee covers the use of ab97783 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/100 - 1/1000.
WB 1/1000 - 1/10000. Predicted molecular weight: 36 kDa.
ICC/IF 1/100 - 1/1000.

靶标

  • 功能Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.
  • 疾病相关Ectodermal dysplasia, anhidrotic, with T-cell immunodeficiency autosomal dominant
  • 序列相似性Belongs to the NF-kappa-B inhibitor family.
    Contains 5 ANK repeats.
  • 翻译后修饰Phosphorylated; disables inhibition of NF-kappa-B DNA-binding activity. Phosphorylation at positions 32 and 36 is prerequisite to recognition by UBE2D3 leading to polyubiquitination and subsequent degradation.
    Sumoylated; sumoylation requires the presence of the nuclear import signal. Sumoylation blocks ubiquitination and proteasome-mediated degradation of the protein thereby increasing the protein stability.
    Monoubiquitinated at Lys-21 and/or Lys-22 by UBE2D3. Ubiquitin chain elongation is then performed by CDC34 in cooperation with the SCF(FBXW11) E3 ligase complex, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. The resulting polyubiquitination leads to protein degradation. Also ubiquitinated by SCF(BTRC) following stimulus-dependent phosphorylation at Ser-32 and Ser-36.
    Deubiquitinated by porcine reproductive and respiratory syndrome virus Nsp2 protein, which thereby interferes with NFKBIA degradation and impairs subsequent NF-kappa-B activation.
  • 细胞定位Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export.
  • Information by UniProt
  • 数据库链接
  • 别名
    • I kappa B alpha antibody
    • I-kappa-B-alpha antibody
    • IkappaBalpha antibody
    • IkB-alpha antibody
    • IKBA antibody
    • IKBA_HUMAN antibody
    • IKBalpha antibody
    • MAD 3 antibody
    • MAD3 antibody
    • Major histocompatibility complex enhancer-binding protein MAD3 antibody
    • NF kappa B inhibitor alpha antibody
    • NF-kappa-B inhibitor alpha antibody
    • NFKBI antibody
    • NFKBIA antibody
    • Nuclear factor of kappa light chain gene enhancer in B cells antibody
    • Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha antibody
    see all

Anti-IKB alpha antibody 图像

  • All lanes : Anti-IKB alpha antibody (ab97783) at 1/5000 dilution

    Lane 1 : BCL-1 whole cell lysate
    Lane 2 : C2C12 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 36 kDa

    10 % SDS-PAGE

  • Anti-IKB alpha antibody (ab97783) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size : 36 kDa
    10% SDS-PAGE.
  • ab97783 at 1/500 dilution detects IKB alpha protein at nucleus on U251 xenograft by immunohistochemical analysis.
    Sample: Paraffin-embedded U251 xenograft.

  • ab97783 at 1/200 dilution staining IKB alpha in HeLa cells by Immunofluorescence, Paraformaldehyde fixed. Lower image is merged with DNA probe.

Anti-IKB alpha antibody (ab97783)参考文献

ab97783 has not yet been referenced specifically in any publications.

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