Anti-IKB alpha抗体(ab97783)
Key features and details
- Rabbit polyclonal to IKB alpha
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-IKB alpha抗体
参阅全部 IKB alpha 一抗 -
描述
兔多克隆抗体to IKB alpha -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Cow, Pig -
免疫原
Recombinant fragment corresponding to Human IKB alpha (internal sequence). a sequence within the center region
Database link: P25963 -
阳性对照
- 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji whole cell lysates; HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab97783于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/100 - 1/1000.
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WB |
1/1000 - 1/10000. Predicted molecular weight: 36 kDa.
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ICC/IF |
1/100 - 1/1000.
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说明 |
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IHC-P
1/100 - 1/1000. |
WB
1/1000 - 1/10000. Predicted molecular weight: 36 kDa. |
ICC/IF
1/100 - 1/1000. |
靶标
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功能
Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription. -
疾病相关
Ectodermal dysplasia, anhidrotic, with T-cell immunodeficiency autosomal dominant -
序列相似性
Belongs to the NF-kappa-B inhibitor family.
Contains 5 ANK repeats. -
翻译后修饰
Phosphorylated; disables inhibition of NF-kappa-B DNA-binding activity. Phosphorylation at positions 32 and 36 is prerequisite to recognition by UBE2D3 leading to polyubiquitination and subsequent degradation.
Sumoylated; sumoylation requires the presence of the nuclear import signal. Sumoylation blocks ubiquitination and proteasome-mediated degradation of the protein thereby increasing the protein stability.
Monoubiquitinated at Lys-21 and/or Lys-22 by UBE2D3. Ubiquitin chain elongation is then performed by CDC34 in cooperation with the SCF(FBXW11) E3 ligase complex, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. The resulting polyubiquitination leads to protein degradation. Also ubiquitinated by SCF(BTRC) following stimulus-dependent phosphorylation at Ser-32 and Ser-36.
Deubiquitinated by porcine reproductive and respiratory syndrome virus Nsp2 protein, which thereby interferes with NFKBIA degradation and impairs subsequent NF-kappa-B activation. -
细胞定位
Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export. - Information by UniProt
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数据库链接
- Entrez Gene: 282291 Cow
- Entrez Gene: 4792 Human
- Entrez Gene: 18035 Mouse
- Entrez Gene: 406188 Pig
- Entrez Gene: 25493 Rat
- Omim: 164008 Human
- SwissProt: P25963 Human
- SwissProt: Q9Z1E3 Mouse
see all -
别名
- I kappa B alpha antibody
- I-kappa-B-alpha antibody
- IkappaBalpha antibody
see all
图片
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All lanes : Anti-IKB alpha antibody (ab97783) at 1/5000 dilution
Lane 1 : BCL-1 whole cell lysate
Lane 2 : C2C12 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 36 kDa10 % SDS-PAGE
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Anti-IKB alpha antibody (ab97783) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 36 kDa
10% SDS-PAGE. -
ab97783 at 1/500 dilution detects IKB alpha protein at nucleus on U251 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded U251 xenograft. -
ab97783 at 1/200 dilution staining IKB alpha in HeLa cells by Immunofluorescence, Paraformaldehyde fixed. Lower image is merged with DNA probe.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab97783 被引用在 4 文献中.
- Chen Y & Sun B PTPRO activates TLR4/NF-κB signaling to intensify lipopolysaccharide-induced pneumonia cell injury. Allergol Immunopathol (Madr) 50:119-124 (2022). PubMed: 35527665
- Chen Z et al. MicroRNA‑129 inhibits colorectal cancer cell proliferation, invasion and epithelial‑to‑mesenchymal transition by targeting SOX4. Oncol Rep 45:N/A (2021). PubMed: 33760159
- Dias AMM et al. Lactobacillus stress protein GroEL prevents colonic inflammation. J Gastroenterol 56:442-455 (2021). PubMed: 33782752
- Nguyen PL et al. Suppression of LPS-Induced Inflammation and Cell Migration by Azelastine through Inhibition of JNK/NF-κB Pathway in BV2 Microglial Cells. Int J Mol Sci 22:N/A (2021). PubMed: 34445767