- 数据表
- 具体的参考文献 (18)
- 实验方案
概述
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产品名称Anti-IKB alpha抗体
参阅全部 IKB alpha 一抗 -
描述兔多克隆抗体to IKB alpha
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宿主Rabbit
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经测试应用适用于: WB, IHC-FrFl, ICC, IHC-P, IHC-Fr, ICC/IF, IPmore details
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种属反应性与反应: Mouse, Rat, Human
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免疫原
Recombinant full length protein (Mouse).
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液Preservative: 0.1% Sodium Azide
Constituents: PBS -
Concentration information loading... -
纯度Protein A purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
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Immunohistochemistry kits
应用
Our Abpromise guarantee covers the use of ab7217 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/2000. | |
| IHC-FrFl | Use at an assay dependent concentration. | |
| ICC | Use at an assay dependent concentration. | |
| IHC-P | 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| IHC-Fr | 1/200. | |
| ICC/IF | 1/200. | |
| IP | Use at 2 µg/mg of lysate. |
靶标
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功能Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.
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疾病相关Ectodermal dysplasia, anhidrotic, with T-cell immunodeficiency autosomal dominant
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序列相似性Belongs to the NF-kappa-B inhibitor family.
Contains 5 ANK repeats. -
翻译后修饰Phosphorylated; disables inhibition of NF-kappa-B DNA-binding activity. Phosphorylation at positions 32 and 36 is prerequisite to recognition by UBE2D3 leading to polyubiquitination and subsequent degradation.
Sumoylated; sumoylation requires the presence of the nuclear import signal. Sumoylation blocks ubiquitination and proteasome-mediated degradation of the protein thereby increasing the protein stability.
Monoubiquitinated at Lys-21 and/or Lys-22 by UBE2D3. Ubiquitin chain elongation is then performed by CDC34 in cooperation with the SCF(FBXW11) E3 ligase complex, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. The resulting polyubiquitination leads to protein degradation. Also ubiquitinated by SCF(BTRC) following stimulus-dependent phosphorylation at Ser-32 and Ser-36.
Deubiquitinated by porcine reproductive and respiratory syndrome virus Nsp2 protein, which thereby interferes with NFKBIA degradation and impairs subsequent NF-kappa-B activation. -
细胞定位Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export.
- Information by UniProt
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数据库链接
- Entrez Gene: 4792 Human
- Entrez Gene: 18035 Mouse
- Entrez Gene: 25493 Rat
- Omim: 164008 Human
- SwissProt: P25963 Human
- SwissProt: Q9Z1E3 Mouse
- SwissProt: Q63746 Rat
- Unigene: 81328 Human
see all -
别名
- I kappa B alpha antibody
- I-kappa-B-alpha antibody
- IkappaBalpha antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IKB alpha antibody (ab7217)Image courtesy of an anonymous Abreview.ab7217 staining IKB alpha in human stomach tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using 10mM citrate buffer pH 6.0. Samples were then blocked with 5% serum for 1 hour at 23°C followed by incubation with the primary antibody, at a 1/200 dilution, for 1 hour at 23°C. An undiluted HRP-conjugated goat anti-rabbit IgG polyclonal was used as secondary antibody. -
Immunoprecipitation - Anti-IKB alpha antibody (ab7217)Image courtesy of an anonymous Abreview.Whole cell lysate from HeLa cells used at total protein input 200µg. Immunoprecipitation step performed using Protein A/G. Diluted at 2µg/mg lysate, 16 hours at 4°C in NETN buffer.
ab7217 used as western blot antibody at a 1/1000 dilution to confirm successful Immunoprecipitation. -
Immunohistochemistry (Frozen sections) - Anti-IKB alpha antibody (ab7217)Image courtesy of an anonymous Abreview.ab7217 staining IKB alpha in human stomach tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with acetone and then blocked with 5% serum for 1 hour at 23°C followed by incubation with the primary antibody at a 1/200 dilution for 1 hour at 23°C. An undiluted HRP-conjugated goat anti-rabbit IgG polyclonal was used as secondary antibody. -
Immunocytochemistry/ Immunofluorescence - Anti-IKB alpha antibody (ab7217)Image courtesy of an anonymous Abreview.ab7217 staining IKB alpha in NIH3T3 mouse fibroblast cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in formaldehyde, permeabilised in 0.025% Triton X and then blocked using 5% serum for 1 hour at 23°C. Samples were then incubated with primary antibody at 1/500 for 1 hour at 23°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 568 (red) used at a 1/1000 dilution.
实验方案
文献
This product has been referenced in:
- Huang N et al. MicroRNA-17-5p aggravates lipopolysaccharide-induced injury in nasal epithelial cells by targeting Smad7. BMC Cell Biol 19:1 (2018). Read more (PubMed: 29433423) »
- Guan G et al. Expression of RNA-binding motif 10 is associated with advanced tumor stage and malignant behaviors of lung adenocarcinoma cancer cells. Tumour Biol 39:1010428317691740 (2017). Read more (PubMed: 28347232) »
客户评价及客户问答
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