概述

  • 产品名称Anti-IKB alpha抗体
    参阅全部 IKB alpha 一抗
  • 描述
    兔多克隆抗体to IKB alpha
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Cow, Human, Pig
    预测可用于: Monkey, Gorilla, Gerbil
  • 免疫原

    Synthetic peptide:

    TESEFTEFTEDELPYDDCVFGGQRLTL

    conjugated to KLH, corresponding to C terminal amino acids 297-317 (with N-terminally added lysine)of Human IKB alpha.

  • 阳性对照
    • whole extract from a human epitheloid carcinoma HeLa cell line. whole extract from the human epidermal carcinoma A431 cell line.

性能

应用

Our Abpromise guarantee covers the use of ab49978 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
AP Use at an assay dependent dilution.
WB 1/2000. Predicted molecular weight: 36 kDa.

靶标

  • 功能Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.
  • 疾病相关Ectodermal dysplasia, anhidrotic, with T-cell immunodeficiency autosomal dominant
  • 序列相似性Belongs to the NF-kappa-B inhibitor family.
    Contains 5 ANK repeats.
  • 翻译后修饰Phosphorylated; disables inhibition of NF-kappa-B DNA-binding activity. Phosphorylation at positions 32 and 36 is prerequisite to recognition by UBE2D3 leading to polyubiquitination and subsequent degradation.
    Sumoylated; sumoylation requires the presence of the nuclear import signal. Sumoylation blocks ubiquitination and proteasome-mediated degradation of the protein thereby increasing the protein stability.
    Monoubiquitinated at Lys-21 and/or Lys-22 by UBE2D3. Ubiquitin chain elongation is then performed by CDC34 in cooperation with the SCF(FBXW11) E3 ligase complex, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. The resulting polyubiquitination leads to protein degradation. Also ubiquitinated by SCF(BTRC) following stimulus-dependent phosphorylation at Ser-32 and Ser-36.
    Deubiquitinated by porcine reproductive and respiratory syndrome virus Nsp2 protein, which thereby interferes with NFKBIA degradation and impairs subsequent NF-kappa-B activation.
  • 细胞定位Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export.
  • Information by UniProt
  • 数据库链接
  • 别名
    • I kappa B alpha antibody
    • I-kappa-B-alpha antibody
    • IkappaBalpha antibody
    • IkB-alpha antibody
    • IKBA antibody
    • IKBA_HUMAN antibody
    • IKBalpha antibody
    • MAD 3 antibody
    • MAD3 antibody
    • Major histocompatibility complex enhancer-binding protein MAD3 antibody
    • NF kappa B inhibitor alpha antibody
    • NF-kappa-B inhibitor alpha antibody
    • NFKBI antibody
    • NFKBIA antibody
    • Nuclear factor of kappa light chain gene enhancer in B cells antibody
    • Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha antibody
    see all

Anti-IKB alpha antibody (ab49978)参考文献

This product has been referenced in:
  • Caperna TJ  et al. Identification and characterization of a nuclear factor-? B-p65 proteolytic fragment in nuclei of porcine hepatocytes in monolayer culture. Domest Anim Endocrinol 45:154-62 (2013). WB ; Pig . Read more (PubMed: 24011531) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Cow Cell lysate - whole cell (macrophage)
Loading amount 10 µg
Specification macrophage
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
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提交于 Apr 07 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"