概述

  • 产品名称Anti-IKB alpha抗体
    参阅全部 IKB alpha 一抗
  • 描述
    兔多克隆抗体to IKB alpha
  • 特异性ab47424 detects endogenous levels of total IKB alpha protein.
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide corresponding to Human IKB alpha. Synthetic non phosphopeptide derived from human IKB alpha around the phosphorylation site of tyrosine 42 (E-E-YP-E-Q Immunogen is in the range of aa 9-58.
    Database link: P25963

  • 阳性对照
    • Human breast carcinoma tissue. 293 cells extract.

性能

应用

Our Abpromise guarantee covers the use of ab47424 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Predicted molecular weight: 35 kDa.
ELISA 1/10000.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能Inhibits the activity of dimeric NF-kappa-B/REL complexes by trapping REL dimers in the cytoplasm through masking of their nuclear localization signals. On cellular stimulation by immune and proinflammatory responses, becomes phosphorylated promoting ubiquitination and degradation, enabling the dimeric RELA to translocate to the nucleus and activate transcription.
  • 疾病相关Ectodermal dysplasia, anhidrotic, with T-cell immunodeficiency autosomal dominant
  • 序列相似性Belongs to the NF-kappa-B inhibitor family.
    Contains 5 ANK repeats.
  • 翻译后修饰Phosphorylated; disables inhibition of NF-kappa-B DNA-binding activity. Phosphorylation at positions 32 and 36 is prerequisite to recognition by UBE2D3 leading to polyubiquitination and subsequent degradation.
    Sumoylated; sumoylation requires the presence of the nuclear import signal. Sumoylation blocks ubiquitination and proteasome-mediated degradation of the protein thereby increasing the protein stability.
    Monoubiquitinated at Lys-21 and/or Lys-22 by UBE2D3. Ubiquitin chain elongation is then performed by CDC34 in cooperation with the SCF(FBXW11) E3 ligase complex, building ubiquitin chains from the UBE2D3-primed NFKBIA-linked ubiquitin. The resulting polyubiquitination leads to protein degradation. Also ubiquitinated by SCF(BTRC) following stimulus-dependent phosphorylation at Ser-32 and Ser-36.
    Deubiquitinated by porcine reproductive and respiratory syndrome virus Nsp2 protein, which thereby interferes with NFKBIA degradation and impairs subsequent NF-kappa-B activation.
  • 细胞定位Cytoplasm. Nucleus. Shuttles between the nucleus and the cytoplasm by a nuclear localization signal (NLS) and a CRM1-dependent nuclear export.
  • Information by UniProt
  • 数据库链接
  • 别名
    • I kappa B alpha antibody
    • I-kappa-B-alpha antibody
    • IkappaBalpha antibody
    • IkB-alpha antibody
    • IKBA antibody
    • IKBA_HUMAN antibody
    • IKBalpha antibody
    • MAD 3 antibody
    • MAD3 antibody
    • Major histocompatibility complex enhancer-binding protein MAD3 antibody
    • NF kappa B inhibitor alpha antibody
    • NF-kappa-B inhibitor alpha antibody
    • NFKBI antibody
    • NFKBIA antibody
    • Nuclear factor of kappa light chain gene enhancer in B cells antibody
    • Nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor alpha antibody
    see all

Anti-IKB alpha antibody 图像

  • All lanes : Anti-IKB alpha antibody (ab47424) at 1/500 dilution

    Lane 1 : extracts from 293 cells treated with TNF alpha (20ng/ml, 30min). No preincupation with immunizing peptide
    Lane 2 : extracts from 293 cells treated with TNF alpha (20ng/ml, 30min). Sample preincubabted with immunizing peptide.


    Predicted band size : 35 kDa
    Observed band size : 35 kDa
  • Immunhistochemical analysis of IKB alpha expression in Paraffin embedded human breast carcinoma tissue sections using 1/50 ab47424. Left: untreated sample. Right: Immunizing peptide treated sample (negative control).

Anti-IKB alpha antibody (ab47424)参考文献

ab47424 has not yet been referenced specifically in any publications.

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