Anti-Ikaros抗体(ab106787)

概述

• 产品名称
  Anti-Ikaros抗体
  参阅全部 Ikaros 一抗
• 描述
  山羊多克隆抗体to Ikaros
• 经测试应用
  适用于: WB, ICCmore details
• 种属反应性
  与反应: Human
  预测可用于: Cow, Dog
• 免疫原

  Synthetic peptide:

  C-DTPYDSSASYEKEN

  , corresponding to internal sequence amino acids 290-303 of Human Ikaros (NP_006051.1)

  Run BLAST with Run BLAST with
• 阳性对照
  • MOLT4 lysate.

性能

• 形式
  Liquid
• 存放说明
  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• 存储溶液
  Preservative: 0.02% Sodium Azide
  Constituents: 0.5% BSA, 0.5mg/ml Tris buffered saline, pH 7.3
• Concentration information loading...
• 纯度
  Immunogen affinity purified
• 纯化说明
  Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• 克隆
  多克隆
• 同种型
  IgG
• 研究领域
  • Epigenetics and Nuclear Signaling
  • Transcription
  • Domain Families
  • Zinc Finger

  • Cancer
  • Invasion/microenvironment
  • Apoptosis
  • Other

  • Cancer
  • Cell Death
  • Apoptosis
  • Other

相关产品

• Compatible Secondaries
  • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
  • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
• Positive Controls
  • MOLT4 whole cell lysate (ab7912)

应用

靶标

• 功能
  Transcription regulator of hematopoietic cell differentiation (PubMed:17934067). Binds gamma-satellite DNA (PubMed:17135265, PubMed:19141594). Plays a role in the development of lymphocytes, B- and T-cells. Binds and activates the enhancer (delta-A element) of the CD3-delta gene. Repressor of the TDT (fikzfterminal deoxynucleotidyltransferase) gene during thymocyte differentiation. Regulates transcription through association with both HDAC-dependent and HDAC-independent complexes. Targets the 2 chromatin-remodeling complexes, NuRD and BAF (SWI/SNF), in a single complex (PYR complex), to the beta-globin locus in adult erythrocytes. Increases normal apoptosis in adult erythroid cells. Confers early temporal competence to retinal progenitor cells (RPCs) (By similarity). Function is isoform-specific and is modulated by dominant-negative inactive isoforms (PubMed:17135265, PubMed:17934067).
• 组织特异性
  Abundantly expressed in thymus, spleen and peripheral blood Leukocytes and lymph nodes. Lower expression in bone marrow and small intestine.
• 疾病相关
  Defects in IKZF1 are frequent occurrences (28.6%) in acute lymphoblasic leukemia (ALL). Such alterations or deletions lead to poor prognosis for ALL.
  Chromosomal aberrations involving IKZF1 are a cause of B-cell non-Hodgkin lymphomas (B-cell NHL). Translocation t(3;7)(q27;p12), with BCL6.
• 序列相似性
  Belongs to the Ikaros C2H2-type zinc-finger protein family.
  Contains 6 C2H2-type zinc fingers.
• 结构域
  The N-terminal zinc-fingers 2 and 3 are required for DNA binding as well as for targeting IKFZ1 to pericentromeric heterochromatin.
  The C-terminal zinc-finger domain is required for dimerization.
• 翻译后修饰
  Phosphorylation controls cell-cycle progression from late G(1) stage to S stage. Hyperphosphorylated during G2/M phase. Dephosphorylated state during late G(1) phase. Phosphorylation on Thr-140 is required for DNA and pericentromeric location during mitosis. CK2 is the main kinase, in vitro. GSK3 and CDK may also contribute to phosphorylation of the C-terminal serine and threonine residues. Phosphorylation on these C-terminal residues reduces the DNA-binding ability. Phosphorylation/dephosphorylation events on Ser-13 and Ser-295 regulate TDT expression during thymocyte differentiation. Dephosphorylation by protein phosphatase 1 regulates stability and pericentromeric heterochromatin location. Phosphorylated in both lymphoid and non-lymphoid tissues (By similarity). Phosphorylation at Ser-361 and Ser-364 downstream of SYK induces nuclear translocation.
  Sumoylated. Simulataneous sumoylation on the 2 sites results in a loss of both HDAC-dependent and HDAC-independent repression. Has no effect on pericentromeric heterochromatin location. Desumoylated by SENP1.
  Polyubiquitinated.
• 细胞定位
  Cytoplasm; Nucleus. In resting lymphocytes, distributed diffusely throughout the nucleus. Localizes to pericentromeric heterochromatin in proliferating cells. This localization requires DNA binding which is regulated by phosphorylation / dephosphorylation events and Nucleus. In resting lymphocytes, distributed diffusely throughout the nucleus. Localizes to pericentromeric heterochromatin in proliferating cells. This localization requires DNA binding which is regulated by phosphorylation / dephosphorylation events (By similarity).

• Target information above from: UniProt accession Q13422 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• 数据库链接
  • Entrez Gene: 10320 Human
  • Omim: 603023 Human
  • SwissProt: Q13422 Human
  • Unigene: 435949 Human
  • Unigene: 488251 Human
  • Unigene: 646004 Human
  • Unigene: 731495 Human
• 形式
  There are 7 isoforms produced by alternative splicing.
• 别名
  • CLL associated antigen KW 6 antibody
  • DNA-binding protein Ikaros antibody
  • hIk 1 antibody

  • Hs.54452 antibody
  • IK1 antibody
  • Ikaros (zinc finger protein) antibody
  • IKAROS antibody
  • IKAROS family zinc finger 1 (Ikaros) antibody
  • Ikaros family zinc finger protein 1 antibody
  • Ikzf1 antibody
  • IKZF1_HUMAN antibody
  • LYF1 antibody
  • Lymphoid transcription factor LyF-1 antibody
  • PRO0758 antibody
  • Zinc finger protein subfamily 1A 1 (Ikaros) antibody
  • Zinc finger protein subfamily 1A 1 antibody
  • Zinc finger protein, subfamily 1A, member 1 antibody
  • ZNFN1A1 antibody

  see all

实验方案

• Western blot protocols
• Immunocytochemistry & immunofluorescence protocols