Anti-IKAP抗体- Carboxyterminal end (ab62498)


  • 产品名称Anti-IKAP抗体- Carboxyterminal end
    参阅全部 IKAP 一抗
  • 描述
    兔多克隆抗体to IKAP - Carboxyterminal end
  • 特异性This antibody reacts with IKAP
  • 经测试应用适用于: WB, ICC, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    A 16 amino acid synthetic peptide from near the carboxy terminus of human IKAP.

  • 阳性对照
    • A20 cell lysate and A20 cells



Our Abpromise guarantee covers the use of ab62498 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 148, 100 kDa (predicted molecular weight: 148 kDa).
ICC Use a concentration of 1 µg/ml.
ICC/IF Use a concentration of 20 µg/ml.


  • 功能May act as a scaffold protein that may assemble active IKK-MAP3K14 complexes (IKKA, IKKB and MAP3K14/NIK).
    Acts as subunit of the RNA polymerase II elongator complex, which is a histone acetyltransferase component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation. Elongator may play a role in chromatin remodeling and is involved in acetylation of histones H3 and probably H4.
  • 疾病相关Defects in IKBKAP are the cause of hereditary sensory and autonomic neuropathy type 3 (HSAN3) [MIM:223900]; also known as Riley-Day syndrome or familial dysautonomia (FD). This autosomal recessive disorder is due to the poor development and survival, and progressive degeneration of the sensory, sympathetic and parasympathetic neurons. HSAN3 individuals are affected with a variety of symptoms such as decreased sensitivity to pain and temperature, cardiovascular instability, recurrent pneumonias, vomiting crises, and gastrointestinal dysfunction. It is primarily confined to individuals of Ashkenazi Jewish descent, with an incidence of 1/3'600 live births.
  • 序列相似性Belongs to the ELP1/IKA1 family.
  • 细胞定位Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp781H1425 antibody
    • DYS antibody
    • Dysautonomia (Riley Day syndrome hereditary sensory autonomic neuropathy type III) antibody
    • Elongator complex protein 1 antibody
    • ELP 1 antibody
    • ELP1 antibody
    • ELP1_HUMAN antibody
    • FD antibody
    • FLJ12497 antibody
    • IKAP antibody
    • IkappaB kinase complex associated protein antibody
    • IkappaB kinase complex-associated protein antibody
    • ikbkap antibody
    • IKI 3 antibody
    • IKI3 antibody
    • IKK complex associated protein antibody
    • IKK complex-associated protein antibody
    • Inhibitor of kappa light polypeptide gene enhancer in B cells kinase complex associated protein antibody
    • OTTHUMP00000063889 antibody
    • p150 antibody
    • TOT 1 antibody
    • TOT1 antibody
    see all

Anti-IKAP antibody - Carboxyterminal end 图像

  • Lane 1 : Anti-IKAP antibody - Carboxyterminal end (ab62498) at 0.5 µg/ml
    Lane 2 : Anti-IKAP antibody - Carboxyterminal end (ab62498) at 1 µg/ml

    Lane 1 : A20 cell lysate
    Lane 2 : A20 cell lysate

    Lysates/proteins at 15 µg per lane.

    anti-rabbit IgG

    Predicted band size : 148 kDa
    Observed band size : 100,148 kDa (why is the actual band size different from the predicted?)
  • Immunocytochemistry of IKAP in A20 cells with ab62498 antibody at 1 µg/ml.
  • Immunofluorescence of IKAP in A20 cells using ab62498 at 20 ug/ml.

Anti-IKAP antibody - Carboxyterminal end (ab62498)参考文献

ab62498 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (testis)
Antigen retrieval step Heat mediated
Permeabilization No
Specification testis
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde

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提交于 Dec 04 2015