概述

  • 产品名称Anti-IKAP抗体
    参阅全部 IKAP 一抗
  • 描述
    小鼠单克隆抗体to IKAP
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment: VLFLFEFDEQ GRELQKAFED TLQLMERSLP EIWTLTYQQN SATPVLGPNS TANSIMASYQ QQKTSVPVLD AELFIPPKIN RRTQWKLSLL , corresponding to amino acids 1242-1332 of Human IKAP

性能

应用

Our Abpromise guarantee covers the use of ab56362 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 150 kDa.
IHC-P Use a concentration of 3 µg/ml.
ICC/IF Use a concentration of 10 µg/ml.

靶标

  • 功能May act as a scaffold protein that may assemble active IKK-MAP3K14 complexes (IKKA, IKKB and MAP3K14/NIK).
    Acts as subunit of the RNA polymerase II elongator complex, which is a histone acetyltransferase component of the RNA polymerase II (Pol II) holoenzyme and is involved in transcriptional elongation. Elongator may play a role in chromatin remodeling and is involved in acetylation of histones H3 and probably H4.
  • 疾病相关Defects in IKBKAP are the cause of hereditary sensory and autonomic neuropathy type 3 (HSAN3) [MIM:223900]; also known as Riley-Day syndrome or familial dysautonomia (FD). This autosomal recessive disorder is due to the poor development and survival, and progressive degeneration of the sensory, sympathetic and parasympathetic neurons. HSAN3 individuals are affected with a variety of symptoms such as decreased sensitivity to pain and temperature, cardiovascular instability, recurrent pneumonias, vomiting crises, and gastrointestinal dysfunction. It is primarily confined to individuals of Ashkenazi Jewish descent, with an incidence of 1/3'600 live births.
  • 序列相似性Belongs to the ELP1/IKA1 family.
  • 细胞定位Cytoplasm. Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZp781H1425 antibody
    • DYS antibody
    • Dysautonomia (Riley Day syndrome hereditary sensory autonomic neuropathy type III) antibody
    • Elongator complex protein 1 antibody
    • ELP 1 antibody
    • ELP1 antibody
    • ELP1_HUMAN antibody
    • FD antibody
    • FLJ12497 antibody
    • IKAP antibody
    • IkappaB kinase complex associated protein antibody
    • IkappaB kinase complex-associated protein antibody
    • ikbkap antibody
    • IKI 3 antibody
    • IKI3 antibody
    • IKK complex associated protein antibody
    • IKK complex-associated protein antibody
    • Inhibitor of kappa light polypeptide gene enhancer in B cells kinase complex associated protein antibody
    • OTTHUMP00000063889 antibody
    • p150 antibody
    • TOT 1 antibody
    • TOT1 antibody
    see all

Anti-IKAP antibody 图像

  • IKAP antibody (ab56362) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human colon.


  • Predicted band size : 150 kDa
    IKAP antibody (ab56362) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • ICC/IF image of ab56362 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56362, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-IKAP antibody (ab56362)参考文献

ab56362 has not yet been referenced specifically in any publications.

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