Mouse, Rat, Sheep, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
Synthetic peptide corresponding to Human IGSF1 aa 151-200 (N terminal).
WB: HepG2 cell lysate
IHC-P: Human kidney tissue
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Protein A purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1.25 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 148 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/312500.
Use a concentration of 4 - 8 µg/ml.
IGSF1 is a member of the immunoglobulin (Ig) superfamily. They have a variety of functions, but all appear to play a role in cell recognition and the regulation of cell behavior.
Isoforms 1 and 2: Membrane; Multipass membrane protein (Potential).
Isoform 3: Secreted (Potential).
Immunoglobulin like domain containing protein 1 antibody
Western blot - IGSF1 antibody (ab66509)
Anti-IGSF1 antibody (ab66509) at 1.25 µg/ml + HepG2 cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size:
Observed band size:
27 kDa (
why is the actual band size different from the predicted?
Additional bands at:
50 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-IGSF1 antibody(ab66509)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling IGSF1 with ab66509 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.
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