概述

  • 产品名称Anti-IGSF1抗体
  • 描述
    兔多克隆抗体to IGSF1
  • 经测试应用适用于: WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Sheep, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide: CNVNILCHGW LQDLVFMLFK EGYAEPVDYQ VPTGTMAIFS IDNLTPEDEG , corresponding to amino acids 146-195 of Human IGSF1

  • 阳性对照
    • WB: HepG2 cell lysate IHC-P: Human kidney tissue

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab66509 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 148 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

IHC-P Use a concentration of 4 - 8 µg/ml.

靶标

  • 相关性IGSF1 is a member of the immunoglobulin (Ig) superfamily. They have a variety of functions, but all appear to play a role in cell recognition and the regulation of cell behavior.
  • 细胞定位Isoforms 1 and 2: Membrane; Multipass membrane protein (Potential). Isoform 3: Secreted (Potential).
  • 数据库链接
  • 别名
    • IGCD1 antibody
    • IGDC1 antibody
    • Immunoglobulin like domain containing protein 1 antibody
    • Immunoglobulin superfamily member 1 antibody
    • InhBP antibody
    • Inhibin binding protein antibody
    • KIAA0364 antibody
    • MGC75490 antibody
    • p120 antibody
    • PGSF2 antibody
    • Pituitary gland specific factor 2 antibody
    see all

Anti-IGSF1 antibody 图像

  • Anti-IGSF1 antibody (ab66509) at 1.25 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 148 kDa
    Observed band size : 27 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling IGSF1 with ab66509 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.

Anti-IGSF1 antibody (ab66509)参考文献

ab66509 has not yet been referenced specifically in any publications.

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