The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.25 - 1 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 95 kDa).
Use a concentration of 20 µg/ml. Heat induced antigen retrieval recommended.
相关性There are four IgG subclasses (IgG1, 2, 3 and 4) in humans, named in order of their abundance in serum (IgG1 being the most abundant). The IgG2a Ig subclass plays a critical role in the pathogenesis of humoral auto-immunity and protection against pathogens.
Anti-IgG2a antibody [10D7A7B2] 图像
Western blot - Anti-IgG2a antibody [10D7A7B2] (ab113683)
All lanes : Anti-IgG2a antibody [10D7A7B2] (ab113683)
Lane 1 : Yeast whole cell lysate Lane 2 : yeast membrane preparation