重组
RabMAb

Anti-IgG1抗体[EPR4417] (ab108969)

概述

  • 产品名称
    Anti-IgG1抗体[EPR4417]
    参阅全部 IgG1 一抗
  • 描述
    兔单克隆抗体[EPR4417] to IgG1
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    corresponding to Human IgG1 (Hinge).

  • 阳性对照
    • Human tonsil and human spleen lysates.
  • 常规说明

     

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.
  • 解离常数(KD
    KD = 3.87 x 10 -10 M Learn more about KD
  • 存储溶液
    pH: 7.40
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 克隆
    单克隆
  • 克隆编号
    EPR4417
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab108969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 36 kDa.

靶标

  • 相关性
    There are four IgG subclasses (IgG1, 2, 3 and 4) in humans, named in order of their abundance in serum (IgG1 being the most abundant).
  • 细胞定位
    Secreted. Cell membrane; Single-pass membrane protein
  • 数据库链接
  • 别名
    • Ig gamma 1 chain C region antibody
    • IgG heavy chain locus antibody
    • IGHG1 antibody
    • Immunoglobulin Gm1 antibody
    • Immunoglobulin heavy constant gamma 1 (G1m marker) antibody
    see all

Anti-IgG1 antibody [EPR4417] 图像

  • All lanes : Anti-IgG1 antibody [EPR4417] (ab108969) at 1/10000 dilution (purified)

    Lane 1 : Human tonsil lysate
    Lane 2 : Human spleen lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size : 36 kDa
    Observed band size : 52 kDa (why is the actual band size different from the predicted?)

    Blocking and diluting buffer: 5% NFDM/TBST.

  • All lanes : Anti-IgG1 antibody [EPR4417] (ab108969) at 1/1000 dilution (unpurified)

    Lane 1 : IgG1 at 0.01 µg
    Lane 2 : IgG2 at 0.01 µg
    Lane 3 : IgG3 at 0.01 µg
    Lane 4 : IgG4 at 0.01 µg
    Lane 5 : Human tonsil lysate at 10 µg
    Lane 6 : Human spleen lysate at 10 µg

    Secondary
    HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size : 36 kDa
  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

实验方案

Anti-IgG1 antibody [EPR4417] (ab108969)参考文献

ab108969 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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