概述

  • 产品名称
  • 描述
    兔多克隆抗体to IgG1
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 154-180 of Human IgG1 (P01857), conjugated to KLH.

  • 阳性对照
    • HL60 cell line lysates

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • 存储溶液
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    This antibody is purified through a protein A column, followed by peptide affinity purification.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab107866 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/500. Predicted molecular weight: 36 kDa.

靶标

  • 相关性
    There are four IgG subclasses (IgG1, 2, 3 and 4) in humans, named in order of their abundance in serum (IgG1 being the most abundant).
  • 细胞定位
    Secreted. Cell membrane; Single-pass membrane protein
  • 数据库链接
  • 别名
    • Ig gamma 1 chain C region antibody
    • IgG heavy chain locus antibody
    • IGHG1 antibody
    • Immunoglobulin Gm1 antibody
    • Immunoglobulin heavy constant gamma 1 (G1m marker) antibody
    see all

图片

  • Anti-IgG1 antibody (ab107866) at 1/100 dilution + HL60 cell line lysates at 35 µg

    Predicted band size : 36 kDa

实验方案

文献

This product has been referenced in:
  • Douglas JN  et al. Antibodies to the RNA Binding Protein Heterogeneous Nuclear Ribonucleoprotein A1 Colocalize to Stress Granules Resulting in Altered RNA and Protein Levels in a Model of Neurodegeneration in Multiple Sclerosis. J Clin Cell Immunol 7:402 (2016). Read more (PubMed: 27375925) »

See 1 Publication for this product

客户评价及客户问答

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Tonsil)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Basic EDTA / Trilogy
Permeabilization
No
Specification
Tonsil
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 22°C
Fixative
Formaldehyde
Username

Fred Clayton

Verified customer

提交于 Mar 28 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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