Highly pure (>98%), recombinant full length IGFBP7 protein (Human).
Lyophilised:Reconstitute with 100µl sterile water for a 1mg/ml solution. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Constituents: 0.5X PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
The antibody was purified by affinity chromatography employing immobilized IGFBP7 matrix. It is sterile filtered.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 31 kDa. Used in conjunction with compatible secondary reagents the detection limit for recombinant IGFBP7 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Use a concentration of 0.5 µg/ml.
Binds IGF-I and IGF-II with a relatively low affinity. Stimulates prostacyclin (PGI2) production. Stimulates cell adhesion.
Okamura J et al. Downregulation of insulin-like growth factor-binding protein 7 in cisplatin-resistant non-small cell lung cancer. Cancer Biol Ther13:148-55 (2012).
Read more (PubMed: 22277373) »
Bunt J et al. Regulation of cell cycle genes and induction of senescence by overexpression of OTX2 in medulloblastoma cell lines. Mol Cancer Res8:1344-57 (2010).
Read more (PubMed: 21047732) »