Anti-IGFBP7抗体(ab36661)

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概述

  • 产品名称
    Anti-IGFBP7抗体
    参阅全部 IGFBP7 一抗
  • 描述
    兔多克隆抗体to IGFBP7
  • 宿主
    Rabbit
  • 特异性
    This antibody reacts with IGFBP7. It does not recognize IGFBPs 1,2,3,4,5,or 6.
  • 经测试应用
    适用于: ELISA, IHC-P, IHC-Frmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Epitope of human IGFBP7.

  • 常规说明
    Dissolved in 400 µl sterile water (a 1/20 dilution). Dilute further in a buffer solution appropriate for the desired application.

性能

应用

Our Abpromise guarantee covers the use of ab36661 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA 1/1000.
IHC-P 1/500.
IHC-Fr 1/500.

靶标

  • 功能
    Binds IGF-I and IGF-II with a relatively low affinity. Stimulates prostacyclin (PGI2) production. Stimulates cell adhesion.
  • 序列相似性
    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 IGFBP N-terminal domain.
    Contains 1 Kazal-like domain.
  • 翻译后修饰
    N-glycosylated.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
    • 别名
      • AGM antibody
      • Angiomodulin antibody
      • FSTL2 antibody
      • IBP-7 antibody
      • IBP7 antibody
      • IBP7_HUMAN antibody
      • IGF binding protein 7 antibody
      • IGF-binding protein 7 antibody
      • IGFBP rP1 antibody
      • IGFBP-7 antibody
      • IGFBP-rP1 antibody
      • IGFBP7 antibody
      • IGFBPRP1 antibody
      • Insulin like growth factor binding protein 7 antibody
      • Insulin-like growth factor-binding protein 7 antibody
      • MAC25 antibody
      • MAC25 protein antibody
      • PGI2 stimulating factor antibody
      • PGI2-stimulating factor antibody
      • Prostacyclin stimulating factor antibody
      • Prostacyclin-stimulating factor antibody
      • PSF antibody
      • RAMSVPS antibody
      • TAF antibody
      • Tumor-derived adhesion factor antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP7 antibody (ab36661)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP7 antibody (ab36661)
      ab36661 (4µg/ml) staining IGFBP7 in human renal cortex using an automated system (DAKO Autostainer Plus). Using this protocol there is cytoplasmic staining of renal tubules.
      Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

    文献

    ab36661 has not yet been referenced specifically in any publications.

    发表研究结果有使用 ab36661？请让我们知道，以便我们可以引用本数据表中的参考文章。

    客户评价及客户问答

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    4 reviews or Q&A

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    Hi John, Thank you very much for your response. I like to get a replacement IGFBP7 antibody (ab74169). ab74169 antibody has same application and everything. Can you please let me know when I’ll be able to get that antibody.

    I have processed your request to receive ab74169 as a replacement. Your new order number is *****. As this is shipping to Canada, I have requested the antibody be shipped on Monday, as if it ships today, it would arrive on Saturday and I was not sure i...

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    We tested on human tissue. We used the Ventana Discovery Chromomap kit from Ventana. I am answering from home and I believe we tried both the Citrate buffer (low pH) and the TRIS/edta buffer (high pH) CC1 and CC2 from Ventana. It was on the automated V...

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    Having reviewed all of the data that you kindly sent, it is clear that ab36661 is not working as stated on the datasheet. As it is covered under our Abpromise I would be happy to send you either a replacement antibody or to process a refund. We do not...

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    Hi John, You have asked some information regarding the IGFBP7 (ab36661) antibody use. I have copied the mail we received from CTAG below as I mentioned earlier.   “I did not get any staining with the IGFBP7. I tested it on Liver, Pancreas, bowel, uteru...

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    Thank you for sending the information. The only other information that would be helpful is if you could confirm the species that the tissues came from, was it human? Also do you have information on the "sensitive polymer kit" and the antigen retrieval ...

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    Hello, The Anti-IGFBP7 antibody (ab36661) we received on Oct 19th is not working (not staining). Can you please replace a IGFBP7 antibody from a different batch. I appreciate your help.   Thanks.   Reagrds,

    Thank you for contacting us. I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly. I am attaching ou...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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