IGFBP6 overexpression 293T裂解液(全细胞) (ab94189)

概述

  • 产品名称IGFBP6 overexpression 293T裂解液(全细胞)
  • 描述
    IGFBP6 overexpression裂解液
  • 常规说明ab94189 is a 293T cell transfected lysate in which Human IGFBP6 has been transiently over-expressed using a pCMV-IGFBP6 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view the preparation notes.
  • 经测试应用适用于: WBmore details

性能

  • 背景Function: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Similarity: Contains 1 IGFBP N-terminal domain. Contains 1 thyroglobulin type-1 domain. PTM: O-linked glycans consist of hexose (probably Gal), N-acetylhexosamine (probably GalNAc) and sialic acid residues. O-glycosylated with core 1 or possibly core 8 glycans. O-glycosylated on one site only in the region AA 143-168 in cerebrospinal fluid.

应用

Our Abpromise guarantee covers the use of ab94189 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution.

IGFBP6 overexpression 293T lysate (whole cell) 图像

  • ab94189 at 15 µg/lane on an SDS-PAGE gel
  • All lanes : Anti-IGFBP6 antibody (ab60718) at 1/500 dilution

    Lane 1 : IGFBP6 overexpression 293T lysate (whole cell) (ab94189)
    Lane 2 : 293T non-transfected Lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

IGFBP6 overexpression 293T lysate (whole cell) (ab94189)参考文献

ab94189 has not yet been referenced specifically in any publications.

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