概述

  • 产品名称Anti-IGFBP4抗体
    参阅全部 IGFBP4 一抗
  • 描述
    山羊多克隆抗体to IGFBP4
  • 特异性Based on ELISA and immunoblotting, this antibody shows less than 1 % cross-reactivity with recombinant human IGFBP1 and no cross reactivity with recombinant human IGFBP2 and recombinant human IGFBP3.
  • 经测试应用适用于: ELISA, WB, Neutralisingmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length protein (Human).

性能

应用

Our Abpromise guarantee covers the use of ab10734 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
WB
Neutralising
  • 应用说明ELISA: Use at a concentration of 0.5 - 1.0 µg/ml. The detection limit for recombinant human IGFBP4 is approximately 0.06 ng/well.
    Neut: Use at an assay dependent dilution.
    WB: Use at a concentration of 0.1 - 0.2 µg/ml. Detects bands of approximately 25 and 32 kDa. The detection limit for recombinant human IGFBP4 is approximately 2 ng/lane and 5 ng/lane under non-reducing and reducing conditions, respectively.

    The Neutralization Dose50 (ND50) for anti human IGFBP4 is approximately 10 to 30 µg/ml in the presence of 0.3 µg/ml of recombinant human IGFBP4 and 14 ng/ml of recombinant human IGF-II using the human breast cancer cell line MCF-7 cells. The ND50 is the concentration of antibody required to yield one half maximal inhibition of the cytokine activity on a responsive cell line, when the cytokine is present at a concentration just high enough to elicit a maximum response. The exact concentration of antibody required to neutralize human IGFBP4 activity is dependent on the cytokine concentration, cell type, growth conditions, and the type of activity studied.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
    • 序列相似性Contains 1 IGFBP N-terminal domain.
      Contains 1 thyroglobulin type-1 domain.
    • 细胞定位Secreted.
    • Information by UniProt
    • 数据库链接
    • 别名
      • AI875747 antibody
      • BP 4 antibody
      • BP4 antibody
      • Deb2 antibody
      • HT29 IGFBP antibody
      • IBP 4 antibody
      • IBP-4 antibody
      • IBP4 antibody
      • IBP4_HUMAN antibody
      • IGF binding protein 4 antibody
      • IGF-binding protein 4 antibody
      • IGF-BP4 antibody
      • IGFBP 4 antibody
      • IGFBP-4 antibody
      • IGFBP4 antibody
      • Insulin Like Growth Factor Binding Protein 4 antibody
      • Insulin-like growth factor-binding protein 4 antibody
      see all

    Anti-IGFBP4 antibody (ab10734)参考文献

    ab10734 has not yet been referenced specifically in any publications.

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