产品名称 Anti-IGFBP3抗体 参阅全部 IGFBP3 一抗
特异性 ab77635 is expected to recognize both reported isoforms (NP_001013416.1 and NP_000589.2).
WB, ELISA more details
Sheep, Human, Pig
Mouse, Rat, Horse, Cow, Dog
C-RYKVDYESQSTDTQN , from the internal region of human IGFBP3 (NP_001013416.1; NP_000589.2).
Human breast cancer lysate.
存放说明 Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
存储溶液 Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3
Concentration information loading...
纯度 Immunogen affinity purified
纯化说明 ab77635 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide.
Immunizing Peptide (Blocking)
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明 Peptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1/64000.
WB: Use at a concentration of 0.02 - 0.06 µg/ml. Predicted molecular weight: 32 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能 IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
组织特异性 Expressed by most tissues. Present in plasma.
序列相似性 Contains 1 IGFBP N-terminal domain. Contains 1 thyroglobulin type-1 domain.
发展阶段 IGFBP3 levels are higher during extrauterine life and peak during puberty.
结构域 The thyroglobulin type-1 domain mediates interaction with HN.
翻译后修饰 Phosphorylation sites are present in the extracelllular medium.
Information by UniProt
Acid stable subunit of the 140 K IGF complex antibody
Binding protein 29 antibody
Binding protein 53 antibody
Anti-IGFBP3 antibody 图像
Western blot - IGFBP3 antibody (ab77635)
All lanes : Anti-IGFBP3 antibody (ab77635) at 0.02 µg/ml Lane 1 : human breast cancer lysate (in RIPA buffer) Lane 2 : human breast cancer lysate (in RIPA buffer) with immunizing peptide Lysates/proteins at 35 µg per lane. Predicted band size : 32 kDa Observed band size : 28 kDa ( why is the actual band size different from the predicted?) Additional bands at : 50 kDa (possible non-specific binding).
Anti-IGFBP3 antibody (ab77635)参考文献
This product has been referenced in:
et al. Conserved genes and pathways in primary human fibroblast strains undergoing replicative and radiation induced senescence. Biol Res 49:34 (2016).
Read more (PubMed: 27464526) »
et al. Antenatal maternal long-term hypoxia: acclimatization responses with altered gene expression in ovine fetal carotid arteries. PLoS One 8:e82200 (2013).
Read more (PubMed: 24367503) »
See all 3 Publications
for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"