概述

  • 产品名称
  • 描述
    兔多克隆抗体to IGFBP3
  • 经测试应用
    适用于: ICC/IF, WB, ELISA, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    KLH-conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S183 of Human IGFBP3

  • 阳性对照
    • IGFBP3-transfected 293 cell lysate

性能

应用

Our Abpromise guarantee covers the use of ab76001 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 10 µg/ml.
WB 1/50 - 1/100. Predicted molecular weight: 32 kDa.
ELISA 1/1000.
IHC-P 1/10 - 1/50.

靶标

  • 功能
    IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
  • 组织特异性
    Expressed by most tissues. Present in plasma.
  • 序列相似性
    Contains 1 IGFBP N-terminal domain.
    Contains 1 thyroglobulin type-1 domain.
  • 发展阶段
    IGFBP3 levels are higher during extrauterine life and peak during puberty.
  • 结构域
    The thyroglobulin type-1 domain mediates interaction with HN.
  • 翻译后修饰
    Phosphorylation sites are present in the extracelllular medium.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Acid stable subunit of the 140 K IGF complex antibody
    • Binding protein 29 antibody
    • Binding protein 53 antibody
    • BP 53 antibody
    • BP53 antibody
    • Growth hormone dependent binding protein antibody
    • IBP 3 antibody
    • IBP-3 antibody
    • IBP3 antibody
    • IBP3_HUMAN antibody
    • IGF binding protein 3 antibody
    • IGF-binding protein 3 antibody
    • IGFBP 3 antibody
    • IGFBP-3 antibody
    • IGFBP3 antibody
    • Insulin Like Growth Factor Binding Protein 3 antibody
    • Insulin-like growth factor binding protein 3 precursor antibody
    • Insulin-like growth factor-binding protein 3 antibody
    see all

图片

  • All lanes : Anti-IGFBP3 antibody (ab76001) at 1/50 dilution

    Lane 1 : Lysates from non-transfected 293 cells
    Lane 2 : Lysates form 293 cells transiently transfected with IGFBP3 gene

    Lysates/proteins at 2 µg per lane.


    Predicted band size : 32 kDa
    Observed band size : 32 kDa
  • ab76001 at 1/50 dilution, staining IGFBP3 in human breast carcinoma tissue section by Immunohistochemistry (Formalin/PFA fixed paraffin-embedded sections). The DAB staining procedure was used for detection.

  • ICC/IF image of ab76001 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76001, 10µg/ml) overnight at +4°C. The secondary antibody (green) was for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

文献

This product has been referenced in:
  • Yang CH  et al. MicroRNA-21 promotes glioblastoma tumorigenesis by down-regulating insulin-like growth factor-binding protein-3 (IGFBP3). J Biol Chem 289:25079-87 (2014). Human . Read more (PubMed: 25059666) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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