概述

  • 产品名称Anti-IGFBP3抗体
    参阅全部 IGFBP3 一抗
  • 描述
    兔多克隆抗体to IGFBP3
  • 特异性Recognizes human IGFBP 3. Slight crossreactivity with other IGF binding proteins: IGFBP 1: ~10%, IGFBP 2: <0.2%, IGFBP 4: <0.2%, IGFBP 5: <0.2%.
  • 经测试应用适用于: WB, IPmore details
  • 种属反应性
    与反应: Human
    不与反应: Rat, Horse, Cow, Pig
  • 免疫原

    Recombinant full length protein (Human IGFBP 3 expressed in CHO cells).

应用

Our Abpromise guarantee covers the use of ab4248 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/250 - 1/1000. Predicted molecular weight: 40 kDa.
IP Use at an assay dependent dilution.

靶标

  • 功能IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
  • 组织特异性Expressed by most tissues. Present in plasma.
  • 序列相似性Contains 1 IGFBP N-terminal domain.
    Contains 1 thyroglobulin type-1 domain.
  • 发展阶段IGFBP3 levels are higher during extrauterine life and peak during puberty.
  • 结构域The thyroglobulin type-1 domain mediates interaction with HN.
  • 翻译后修饰Phosphorylation sites are present in the extracelllular medium.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Acid stable subunit of the 140 K IGF complex antibody
    • Binding protein 29 antibody
    • Binding protein 53 antibody
    • BP 53 antibody
    • BP53 antibody
    • Growth hormone dependent binding protein antibody
    • IBP 3 antibody
    • IBP-3 antibody
    • IBP3 antibody
    • IBP3_HUMAN antibody
    • IGF binding protein 3 antibody
    • IGF-binding protein 3 antibody
    • IGFBP 3 antibody
    • IGFBP-3 antibody
    • IGFBP3 antibody
    • Insulin Like Growth Factor Binding Protein 3 antibody
    • Insulin-like growth factor binding protein 3 precursor antibody
    • Insulin-like growth factor-binding protein 3 antibody
    see all

Anti-IGFBP3 antibody (ab4248)参考文献

This product has been referenced in:
  • Walker G  et al. Insulin-like growth factor binding proteins IGFBP3, IGFBP4, and IGFBP5 predict endocrine responsiveness in patients with ovarian cancer. Clin Cancer Res 13:1438-44 (2007). Read more (PubMed: 17332286) »

See 1 Publication for this product

Product Wall

Thank you for contacting us. We are happy to provide quote for 400 micro-litre of this antibody. The concentration of this antibody was not determined so we will be unable to give you quote for 400 µg. We generally do not determine the concentratio...

Read More
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (fibroblast)
Specification fibroblast
Fixative Formaldehyde
Permeabilization Yes - 0.5% triton for 5 min
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Jan 18 2008

Thank you for your patience in awaiting a response. Having discussed this issue with my colleagues and the originator of the product. The following may explain the route cause of the problem I have been told that in all cases, the IGFBP's are "stick...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"