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Our Abpromise guarantee covers the use of ab36529 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab36529 staining human fibroblast cells by ICC/IF. Cells were fixed in formaldehyde, permeabilised in 0.5% triton for 5 min, and blocked with 4% BSA for 1 hour at 20°C. The primary antibody was diluted 1/200 with 4% BSA/TBST and incubated for 2 hours at 20°C. An Alexa Fluor® 594 conjugated donkey anti-mouse was used as the secondary antibody.
Red fluorescence shows IGFBP3, blue is DNA.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"