概述

  • 产品名称Anti-IGFBP3抗体
    参阅全部 IGFBP3 一抗
  • 描述
    小鼠单克隆抗体to IGFBP3
  • 特异性Ab36529 is specific to IGFBP3. It shows less than 0.05% reactivity with human IGFBPs 1, 2, 4, 5 or 6, as determined by EIA.
  • 经测试应用适用于: ELISA, WB, Dot blotmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    IGFBP3 plus epitope of human IGFBP3.

性能

应用

Our Abpromise guarantee covers the use of ab36529 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA
WB
Dot blot
  • 应用说明Dot: 1/5000.
    ELISA: 1/8000.
    WB: A 1/5000 dilution detects 60ng IGFBP3. Predicted molecular weight: 32 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.
    • 组织特异性Expressed by most tissues. Present in plasma.
    • 序列相似性Contains 1 IGFBP N-terminal domain.
      Contains 1 thyroglobulin type-1 domain.
    • 发展阶段IGFBP3 levels are higher during extrauterine life and peak during puberty.
    • 结构域The thyroglobulin type-1 domain mediates interaction with HN.
    • 翻译后修饰Phosphorylation sites are present in the extracelllular medium.
    • 细胞定位Secreted.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Acid stable subunit of the 140 K IGF complex antibody
      • Binding protein 29 antibody
      • Binding protein 53 antibody
      • BP 53 antibody
      • BP53 antibody
      • Growth hormone dependent binding protein antibody
      • IBP 3 antibody
      • IBP-3 antibody
      • IBP3 antibody
      • IBP3_HUMAN antibody
      • IGF binding protein 3 antibody
      • IGF-binding protein 3 antibody
      • IGFBP 3 antibody
      • IGFBP-3 antibody
      • IGFBP3 antibody
      • Insulin Like Growth Factor Binding Protein 3 antibody
      • Insulin-like growth factor binding protein 3 precursor antibody
      • Insulin-like growth factor-binding protein 3 antibody
      see all

    Anti-IGFBP3 antibody 图像

    • ab36529 staining human fibroblast cells by ICC/IF.  Cells were fixed in formaldehyde, permeabilised in 0.5% triton for 5 min, and blocked with 4% BSA for 1 hour at 20°C.  The primary antibody was diluted 1/200 with 4% BSA/TBST and incubated for 2 hours at 20°C.  An Alexa Fluor® 594 conjugated donkey anti-mouse was used as the secondary antibody.

      Red fluorescence shows IGFBP3, blue is DNA.

      See Abreview

    Anti-IGFBP3 antibody (ab36529)参考文献

    This product has been referenced in:
    • Chenau J  et al. The cell line secretome, a suitable tool for investigating proteins released in vivo by tumors: application to the study of p53-modulated proteins secreted in lung cancer cells. J Proteome Res 8:4579-91 (2009). WB ; Human . Read more (PubMed: 19639960) »
    • Heath KE  et al. Primary acid-labile subunit deficiency due to recessive IGFALS mutations results in postnatal growth deficit associated with low circulating insulin growth factor (IGF)-I, IGF binding protein-3 levels, and hyperinsulinemia. J Clin Endocrinol Metab 93:1616-24 (2008). Read more (PubMed: 18303074) »

    See all 2 Publications for this product

    Product Wall

    I received some information regarding the immunogen of this product and thought that you might be interested in knowing. The synthetic peptide used as an immunogen for this antibody was derived from the central domain of IGFBP-3 and does not contai...

    Read More
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (fibroblast)
    Specification fibroblast
    Fixative Formaldehyde
    Permeabilization Yes - 0.5% triton for 5 min
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 20°C
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    提交于 Jan 09 2008

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"