概述

  • 产品名称Anti-IGFBP1抗体
    参阅全部 IGFBP1 一抗
  • 描述
    兔多克隆抗体to IGFBP1
  • 经测试应用适用于: IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide derived from an internal region of Human IGFBP1.

  • 阳性对照
    • Human lung carcinoma tissue. IF/ICC: HeLa cell line.

性能

应用

Our Abpromise guarantee covers the use of ab111203 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration.
  • 序列相似性Contains 1 IGFBP N-terminal domain.
    Contains 1 thyroglobulin type-1 domain.
  • 翻译后修饰Phosphorylated; probably by casein kinase II. Alters the affinity of the protein for IGFs.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AFBP antibody
    • Alpha pregnancy associated endometrial globulin antibody
    • Amniotic fluid binding protein antibody
    • Binding protein 25 antibody
    • Binding protein 26 antibody
    • Binding protein 28 antibody
    • Growth hormone independent binding protein antibody
    • hIGFBP 1 antibody
    • hIGFBP1 antibody
    • IBP 1 antibody
    • IBP-1 antibody
    • IBP1 antibody
    • IBP1_HUMAN antibody
    • IGF binding protein 1 antibody
    • IGF BP25 antibody
    • IGF-binding protein 1 antibody
    • IGFBP 1 antibody
    • IGFBP-1 antibody
    • IGFBP1 antibody
    • Insulin like growth factor binding protein 1 antibody
    • Insulin-like growth factor-binding protein 1 antibody
    • Placental protein 12 antibody
    • PP 12 antibody
    • PP12 antibody
    see all

Anti-IGFBP1 antibody 图像

  • ab111203 stained HepG2 cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab111203 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • ab111203 at a 1/50 dilution, staining IGFBP1 in paraffin-embedded Human lung carcinoma tissue by Immunohistochemsitry, in the absence (left) and presence (right) of the immunizing peptide.

Anti-IGFBP1 antibody (ab111203)参考文献

This product has been referenced in:
  • Dai B  et al. Insulin-like growth factor binding protein-1 inhibits cancer cell invasion and is associated with poor prognosis in hepatocellular carcinoma. Int J Clin Exp Pathol 7:5645-54 (2014). Read more (PubMed: 25337205) »

See 1 Publication for this product

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