Anti-IGFBP1抗体[33627.11] (ab10732)

概述

性能

应用

Our Abpromise guarantee covers the use of ab10732 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
Neutralising
  • 应用说明ELISA: Use at a concentration of 2 µg/ml. In the ELISA capture assay, plates are coated with 100 µl/well of the capture antibody (2 µg/ml) in combination with 100 µl/well of a detection antibody (affinity-purified biotinylated polyclonal anti-human IGFBP1 antibody at 100 ng/ml). An ELISA range of 62.4 to 4000 pg/ml may be obtained.
    Neut: Use at a concentration of 10 - 40 µg/ml. The Neutralization Dose50 (ND50) for this antibody is 10-40 µg/ml in the presence of approximately 5 µg/ml of recombinant human IGFBP1 and approximately 6 ng/mL of recombinant human IGF1, using human MCF7 cells.
    WB: Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 28 kDa. The detection limit for recombinant human IGFBP1 is approximately 0.5 ng/lane under non-reducing conditions.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration.
    • 序列相似性Contains 1 IGFBP N-terminal domain.
      Contains 1 thyroglobulin type-1 domain.
    • 翻译后修饰Phosphorylated; probably by casein kinase II. Alters the affinity of the protein for IGFs.
    • 细胞定位Secreted.
    • Information by UniProt
    • 数据库链接
    • 别名
      • AFBP antibody
      • Alpha pregnancy associated endometrial globulin antibody
      • Amniotic fluid binding protein antibody
      • Binding protein 25 antibody
      • Binding protein 26 antibody
      • Binding protein 28 antibody
      • Growth hormone independent binding protein antibody
      • hIGFBP 1 antibody
      • hIGFBP1 antibody
      • IBP 1 antibody
      • IBP-1 antibody
      • IBP1 antibody
      • IBP1_HUMAN antibody
      • IGF binding protein 1 antibody
      • IGF BP25 antibody
      • IGF-binding protein 1 antibody
      • IGFBP 1 antibody
      • IGFBP-1 antibody
      • IGFBP1 antibody
      • Insulin like growth factor binding protein 1 antibody
      • Insulin-like growth factor-binding protein 1 antibody
      • Placental protein 12 antibody
      • PP 12 antibody
      • PP12 antibody
      see all

    Anti-IGFBP1 antibody [33627.11] (ab10732)参考文献

    This product has been referenced in:
    • Walker G  et al. Insulin-like growth factor binding proteins IGFBP3, IGFBP4, and IGFBP5 predict endocrine responsiveness in patients with ovarian cancer. Clin Cancer Res 13:1438-44 (2007). Read more (PubMed: 17332286) »

    See 1 Publication for this product

    Product Wall

    Thank you for your reply.

    In that case, you might want to try one of the other IGFBP1 antibodies to see if they inhibit IGF-1 binding. What applicationhave you been using the antibody ab128423 in or what type of experiment had you optimized ...

    Read More

    Thank you for your emails.

    Ab6302 is a beta-catenin antibody. Unfortunately, I do not have information aboutwhere beta-catenin would bind to IGFBP1.

    Also, I checked the database entry for human IGFBP1 (http://www.uniprot.org/unipro...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"