RabMAb

Anti-IGF2BP2抗体[EPR6741(B)] (ab124930)

概述

  • 产品名称Anti-IGF2BP2抗体[EPR6741(B)]
    参阅全部 IGF2BP2 一抗
  • 描述
    兔单克隆抗体[EPR6741(B)] to IGF2BP2
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human IGF2BP2.

  • 阳性对照
    • 293T, Caco2, HepG2, and SH SY5Y cell lysates, rat brain and mouse heart lysate; Human colon and human lung carcinoma tissue.
  • 常规说明

    The mouse and rat recommendation is based on the WB results. This antibody may not be suitable for IHC with mouse or rat samples.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

性能

应用

Our Abpromise guarantee covers the use of ab124930 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/2000. Detects a band of approximately 55-65 kDa (predicted molecular weight: 66 kDa).
IHC-P 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

ICC/IF 1/100 - 1/250.

靶标

  • 功能Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binding is isoform-specific. May regulate translation of target mRNAs.
  • 组织特异性Expressed in oocytes, granulosa cells of small and growing follicles, Leydig cells, spermatogonia and semen (at protein level). Expressed in testicular cancer (at protein level). Expressed weakly in heart, placenta, skeletal muscle, bone marrow, colon, kidney, salivary glands, testis and pancreas. Detected in fetal liver, fetal ovary, gonocytes and interstitial cells of the testis.
  • 序列相似性Belongs to the RRM IMP/VICKZ family.
    Contains 4 KH domains.
    Contains 2 RRM (RNA recognition motif) domains.
  • 结构域The third and fourth KH domains are important for binding to the untranslated region (UTR) of target mRNA.
  • 细胞定位Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Localizes at the connecting piece and the tail of the spermatozoa.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Hepatocellular carcinoma autoantigen p62 antibody
    • IF2B2_HUMAN antibody
    • IGF 2 mRNA binding protein 2 antibody
    • IGF II mRNA binding protein 2 antibody
    • IGF-II mRNA-binding protein 2 antibody
    • IGF2 mRNA-binding protein 2 antibody
    • Igf2bp2 antibody
    • IMP 2 antibody
    • IMP-2 antibody
    • IMP2 antibody
    • Insulin like growth factor 2 mRNA binding protein 2 antibody
    • Insulin-like growth factor 2 mRNA-binding protein 2 antibody
    • OTTHUMP00000082606 antibody
    • OTTHUMP00000082607 antibody
    • OTTHUMP00000082608 antibody
    • p62 antibody
    • VICKZ 2 antibody
    • VICKZ family member 2 antibody
    • VICKZ2 antibody
    see all

Anti-IGF2BP2 antibody [EPR6741(B)] 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue sections labeling IGF2BP2 with purified ab124930 at 1/200 dilution (4.8 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. Hematoxylin was used to counter stain. ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody at 1/0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Immunocytochemistry/ Immunofluorescence analysis of HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling IGF2BP2 with purified ab124930 at 1/250 dilution (3.8μg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889, an anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 μg/ml). ab150077, a Goat anti-rabbit IgG (Alexa Fluor® 488) was used as the secondary antibody at 1/1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Anti-IGF2BP2 antibody [EPR6741(B)] (ab124930) at 1/10000 dilution (purified) + Mouse heart lysate at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size : 66 kDa
    Observed band size : 66 kDa

    Blocking and diluting buffer: 5% NFDM/TBST.

  • All lanes : Anti-IGF2BP2 antibody [EPR6741(B)] (ab124930) at 1/2000 dilution (purified)

    Lane 1 : 293T (Human embryonic kidney epithelial cell) whole cell lysate
    Lane 2 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate
    Lane 3 : Rat brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size : 66 kDa
    Observed band size : 66 kDa

    Blocking and diluting buffer: 5% NFDM/TBST.

  • All lanes : Anti-IGF2BP2 antibody [EPR6741(B)] (ab124930) at 1/1000 dilution (unpurified)

    Lane 1 : 293T cell lysate
    Lane 2 : Caco2 cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : SH-SY5Y cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP-conjugated goat anti-rabbit at 1/2000 dilution

    Predicted band size : 66 kDa
  • ab124930 (unpurified), at 1/50 dilution, staining GF2BP2 in formalin-fixed, paraffin-embedded Human colon tissue by immunohistochemistry.

  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

Anti-IGF2BP2 antibody [EPR6741(B)] (ab124930)参考文献

ab124930 has not yet been referenced specifically in any publications.

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