- 数据表
- 具体的参考文献 (6)
- 实验方案
概述
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产品名称Anti-IGF2BP1抗体
参阅全部 IGF2BP1 一抗 -
描述兔多克隆抗体to IGF2BP1
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经测试应用适用于: IHC-P, WBmore details
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种属反应性与反应: Dog, Human
预测可用于: Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Zebrafish
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免疫原
Synthetic peptide corresponding to a region within N terminal amino acids 36 - 85 (KSGYAFVDCP DEHWAMKAIE TFSGKVELQG KRLEIEHSVP KKQRSRKIQI) of human IGF2BP1 (NP_006537).
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阳性对照
- Jurkat cell lysate
性能
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形式Liquid
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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存储溶液Preservative: None
Constituents: 2% Sucrose, PBS -
Concentration information loading... -
纯度Immunogen affinity purified
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纯化说明ab82968 is purified by a peptide affinity chromatography method.
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Positive Controls
应用
Our Abpromise guarantee covers the use of ab82968 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | 1/300. | |
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 63 kDa.Can be blocked with Human IGF2BP1 peptide (ab100852). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. |
靶标
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功能RNA-binding factor that affects mRNA nuclear export, localization, stability and translation. Component of the CRD-mediated complex that promotes MYC mRNA stabilization. Regulates mRNA stability during the integrated cellular stress response (ISR) in stress granules (SGs). Stabilizes the BTRC/FBW1A mRNA from degradation by disrupting miRNA-dependent interaction with AGO2. Identified in a HCV IRES-mediated translation complex, that enhances translation at the Hepatitis C virus (HCV) RNA-replicon via the internal ribosome entry site (IRES), but does not affect 5'cap-dependent translation. Acts as a HIV-1 retrovirus restriction factor that reduces HIV-1 assembly by inhibiting viral RNA packaging, assembly and processing of HIV-1 GAG protein on cellular membranes. Binds to mRNAs in stress granules (SGs). Binds to the stem-loop IV of the 5'-UTR and to the variable region and the poly(U-C) motif of the 3'-UTR of the HCV RNA-replicon. Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNA and regulates its subcellular localization and translation. Binds both to the coding region mRNA stability determinant (CRD) and to AU-rich sequences in the 3'-UTR of the MYC and CD44 mRNAs and stabilizes these mRNAs. Binds to the fourth and fifth exons of the oncofetal H19 and neuron-specific TAU mRNAs and regulates their localizations. Binds to the adenine-rich autoregulatory sequence (ARS) 5'-UTR of the PABPC1 mRNA and is involved in its translational repression. The RNA-binding activity to ARS is stimulated by PABPC1. Binds to the coding sequence region of BTRC/FBW1A mRNA and mediates stabilization of BTRC/FBW1A and MYC mRNAs in response to beta-catenin signaling. Binding to RNA employs a cooperative, sequential mechanism of homo- or heterodimerisation. Also involved in growth or survival of lung-cancer cells. Protects the MYC and MDR-1 mRNAs from cleavage by a endoribonuclease, thus prolonging their stabilities (By similarity). Binds to the 3'-UTR axonal localization signal (ALS) of TAU mRNA (By similarity). Binds to a conserved 54-nucleotide element in the 3'-UTR of the beta actin mRNA known as the 'zipcode' (By similarity). Promotes translocation of the beta-actin mRNA to dendrites (By similarity). May act as a regulator of mRNA transport to activated synapses in response to synaptic activity.
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组织特异性Expressed in fetal liver, fetal lung, fetal kidney, fetal thymus, fetal placenta, fetal follicles of ovary, gonocytes of testis, oocytes, spermatogonia and semen (at protein level). Expressed in testicular and lung cancer (at protein level). Expressed in kidney, prostate, trachea, testis and lung cancer.
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序列相似性Belongs to the RRM IMP/VICKZ family.
Contains 4 KH domains.
Contains 2 RRM (RNA recognition motif) domains. -
结构域The third and fourth KH domains encompass the protein dimerization motif and are necessary and sufficient for RNA binding. The four KH domains are important for granule formation and SGs targeting. Contains two nuclear export signals, situated within the second and fourth KH domains. The four KH domains are important to suppress HIV-1 infectivity.
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翻译后修饰Phosphorylated. Phosphorylation may influence mRNA translation.
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细胞定位Nucleus. Cytoplasm. Cell projection > lamellipodium. Cell projection > dendrite. Cell projection > dendritic spine. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Targeted to stress granules (SGs), but not processing bodies (PBs), during cellular stress. Colocalizes with G3BP1 and TIAL1 in SGs. Colocalizes with HIV-1 GAG at the cell edges. Found in lamellipodia of the leading edge, in the perinuclear region, and beneath the plasma membrane. The subcytoplasmic localization is cell specific and regulated by cell contact and growth. Colocalized with H19 RNA at lamellipodia. Colocalized with CD44 mRNA in RNP granules. Nuclear export is mediated by XPO1/CRM1. In motile cells, is transported towards the leading edge into the cortical region of the lamellipodia where it is connected to microfilaments (By similarity). Present in the form of granules and into F-actin-rich protrusion of dendrites, spines and subsynaptic sites (By similarity). Colocalizes with beta-actin mRNA in dendrites and spines (By similarity). Exhibited rapid, bidirectional movements in dendrites and spines (By similarity). Neuronal depolarization by KCl induces its rapid efflux from the cell body into dendrites.
- Information by UniProt
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数据库链接
- Entrez Gene: 395953 Chicken
- Entrez Gene: 516853 Cow
- Entrez Gene: 491064 Dog
- Entrez Gene: 100055918 Horse
- Entrez Gene: 10642 Human
- Entrez Gene: 140486 Mouse
- Entrez Gene: 303477 Rat
- Entrez Gene: 558182 Zebrafish
see all -
别名
- Coding region determinant-binding protein antibody
- CRD BP antibody
- CRD-BP antibody
see all
Anti-IGF2BP1 antibody 图像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF2BP1 antibody (ab82968)Sample Type : Human lung carcinoma. Primary Antibody Dilution : 1:300. Secondary Antibody : Anti-rabbit-linker. Fbex-HRP. Color/Signal Descriptions : Brown: IGFBP1 Blue: Nuclei. Gene Name : IGF2BP1. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF2BP1 antibody (ab82968)Application: IHC. Species+tissue/cell type: Human small intestine. Primary antibody dilution: 1:300. Secondary antibody: Anti-rabbit-linker. Fbex-HRP -
Western blot - Anti-IGF2BP1 antibody (ab82968)Anti-IGF2BP1 antibody (ab82968) at 1 µg/ml (in 5% skim milk / PBS buffer) + jurkat cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 63 kDa
Observed band size : 65 kDa (why is the actual band size different from the predicted?) -
Western blot - Anti-IGF2BP1 antibody (ab82968)This image is courtesy of an Abreview submitted by Liza O'DonoghueLanes 1 & 4 : Anti-IGF2BP1 antibody (ab82968) at 1/1000 dilution (in 5% milk for 1 1/2 hours at 25°C )
Lanes 2 & 5 : Anti-IGF2BP1 antibody (ab82968) at 1/1000 dilution (in 0.5% milk for 1 1/2 hours at 25°C )
Lanes 3 & 6 : Anti-IGF2BP1 antibody (ab82968) at 1/10000 dilution (in 0.5% milk for 1 1/2 hours at 25°C )
Lane 1 : Whole cell lysate of Dog Osteosarcoma (high-expressing cell lines) blocked with 5% milk in TBST for 15 hours at 4°C
Lane 2 : Whole cell lysate of Dog Osteosarcoma (high-expressing cell lines) blocked with 0.5% milk in TBST for 15 hours at 4°C
Lane 3 : Whole cell lysate of Dog Osteosarcoma (high-expressing cell lines) blocked with 0.5% milk in TBST for 15 hours at 4°C
Lane 4 : Whole cell lysate of Dog Osteosarcoma (low-expressing cell lines) blocked with 5% milk in TBST for 15 hours at 4°C
Lane 5 : Whole cell lysate of Dog Osteosarcoma (low-expressing cell lines) blocked with 0.5% milk in TBST for 15 hours at 4°C
Lane 6 : Whole cell lysate of Dog Osteosarcoma (low-expressing cell lines) blocked with 0.5% milk in TBST for 15 hours at 4°C
Lysates/proteins at 50 µg per lane.
Secondary
An HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/10000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 63 kDa
Observed band size : 65 kDa (why is the actual band size different from the predicted?)
Exposure time : 28 minutesThis image is courtesy of an Abreview submitted by Liza O'Donoghue
No bands were visible at the higher dilution, even with a stronger detection substrate. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGF2BP1 antibody (ab82968)Image courtesy of Ms Liza O'Donoghue by Abreview.ab82968 staining IGF2BP1 in dog kidney, sebaceous gland, and epidermis by Immunohistochemistry (paraffin embedded sections). Tissue was fixed with formalin, an antigen retrieval step was performed using a pH6 antigen retrieval solution. Tissue was then blocked followed by incubation with the primary antibody, at a 1/500 dilution, for 16 hours at 4°C. A Biotin-conjugated goat polyclonal was used as secondary antibody at a 1/1000 dilution.
Note that the brown staining in the epidermis section minus primary antibody is natural pigment, not inapropriate antibody binding.
Anti-IGF2BP1 antibody (ab82968)参考文献
This product has been referenced in:
- Fakhraldeen SA et al. Two Isoforms of the RNA Binding Protein, Coding Region Determinant-binding Protein (CRD-BP/IGF2BP1), Are Expressed in Breast Epithelium and Support Clonogenic Growth of Breast Tumor Cells. J Biol Chem 290:13386-400 (2015). WB, ICC/IF ; Mouse . Read more (PubMed: 25861986) »
- Li W et al. Role of IGF2BP3 in trophoblast cell invasion and migration. Cell Death Dis 5:e1025 (2014). WB ; Human . Read more (PubMed: 24457969) »
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