概述

  • 产品名称Anti-IGF2抗体
    参阅全部 IGF2 一抗
  • 描述
    兔多克隆抗体to IGF2
  • 经测试应用适用于: WB, ELISA, Neutralising, ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Cow, Human
  • 免疫原

    Highly pure (>98%) recombinant hIGF-2 (human Insulin Like Growth Factor-2).

  • 阳性对照
    • ab9575 (Active IGF2 full length protein) can be used as a positive control.
  • 常规说明


    Insulin Like Growth Factor-2 (IGF-2) is a polypeptide growth factor, which stimulates the proliferation of a wide range of cell types

性能

应用

Our Abpromise guarantee covers the use of ab9574 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. To detect hIGF-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIGF-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
ELISA Use at an assay dependent concentration. To detect hIGF-2 by ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIGF-2.
Neutralising Use at an assay dependent concentration. To yield one-half maximal inhibition of the biological activity of hIGF-2 (10.0 ng/ml), a concentration of 3.1 - 4.6 µg/ml of this antibody is required.
ICC/IF Use a concentration of 1 - 5 µg/ml.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.
    Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3.
  • 疾病相关Epigenetic changes of DNA hypomethylation in IGF2 are a cause of Silver-Russell syndrome (SIRS) [MIM:180860]. SIRS is a clinically heterogeneous condition characterized by severe intrauterine growth retardation, poor postnatal growth, craniofacial features such as a triangular shaped face and a broad forehead, body asymmetry, and a variety of minor malformations.
  • 序列相似性Belongs to the insulin family.
  • 翻译后修饰O-glycosylated with a core 1 or possibly core 8 glycan.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C11orf43 antibody
    • IGF 2 antibody
    • IGF II antibody
    • IGF-II antibody
    • IGF2 antibody
    • IGF2_HUMAN antibody
    • IGFII antibody
    • INSIGF antibody
    • insulin like growth factor 2 (somatomedin A) antibody
    • Insulin like Growth Factor 2 antibody
    • Insulin like growth factor II antibody
    • Insulin like growth factor II precursor antibody
    • Insulin like growth factor type 2 antibody
    • pp9974 antibody
    • Preptin antibody
    • putative insulin like growth factor II associated protein antibody
    • Somatomedin A antibody
    • Somatomedin-A antibody
    see all

Anti-IGF2 antibody 图像

  • Immunohistochemical analysis of parafin embedded rat kidney tissue labelling IGF2 with ab9574 at 1ug/ml. 

    See Abreview

  • Anti-IGF2 antibody (ab9574) at 1/2000 dilution + whole tissue lysate prepared from murine liver at 40 µg

    Secondary
    HRP conjugated goat anti-rabbit polyclonal at 1/4000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 20 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 minutes

    Image courtesy of an anonymous Abreview.

    See Abreview

  • ICC/IF image of ab9574 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9574, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Rabbit Anti-Human IGF-II against Human IGF-II. Antibody concentration: 0.10 ug/ml Lower limit of detection: 3.91 ng/lane Molecular Weight: 7.5 kDa
    Lane 1: Marker
    Lane 2: 250 ng
    Lane 3: 125 ng
    Lane 4: 62.5 ng
    Lane 5: 31.25 ng
    Lane 6: 15.625 ng
    Lane 7: 7.8 ng
    Lane 8: 3.9 ng

     

  •  ab9574 staining human tongue carcinoma tissue sections by IHC-P. For antigen retrieval sections were placed in a bath of 0.01M citric acid, pH6, and were heated on full power in a microwave for 5 minutes and then for a further 4 minutes. The slides were then cooled and placed in PBS, fixed with paraformaldehyde and blocked with BSA. The slides were incubated with the antibody for 18 hours. An HRP conjugated pig polyclonal antibody was used as the secondary.

    See Abreview

  • IHC-P of normal human placenta stained with ab9574. The recommended concentration ranges from 0.5 µg/ml-5.0 µg/ml.  An HRP-labeled polymer detection system was used with DAB chromogen.  Recommended antigen retrievals are heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer or enzyme induced antigen retrieval with Proteinase K.  Optimal concentrations and conditions may vary.

Anti-IGF2 antibody (ab9574)参考文献

This product has been referenced in:
  • Houlihan SL  et al. Upregulation of neurovascular communication through filamin abrogation promotes ectopic periventricular neurogenesis. Elife 5:N/A (2016). IHC-Fr ; Mouse . Read more (PubMed: 27664421) »
  • Kan SH  et al. Delivery of an enzyme-IGFII fusion protein to the mouse brain is therapeutic for mucopolysaccharidosis type IIIB. Proc Natl Acad Sci U S A 111:14870-5 (2014). Mouse . Read more (PubMed: 25267636) »

See all 28 Publications for this product

Product Wall

Application Immunocytochemistry
Sample Human Cultured Cells (PULP)
Permeabilization Yes - tritonx100
Specification PULP
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative Paraformaldehyde
Username

Ms. kyungjoo seong

Verified customer

提交于 Nov 30 2016

Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Denaturing (10% SDS PAGE)
Sample Mouse Tissue lysate - whole (Liver)
Specification Liver
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jun 02 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Sample Mouse Cell (primary hepatocytes)
Specification primary hepatocytes
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Oct 01 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 50 µg
Gel Running Conditions Non-reduced Non-Denaturing (Native) (15%)
Sample Mouse Serum (primary hepatoctyes)
Specification primary hepatoctyes
Blocking step I-Block(Applied biosystems) as blocking agent for 30 minute(s) · Concentration: 2µg/mL · Temperature: 22°C
Username

Abcam user community

Verified customer

提交于 Jul 02 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Kidney)
Specification Kidney
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: pH 6 Citrate
Permeabilization No
Blocking step DAKO PB as blocking agent for 15 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Abcam user community

Verified customer

提交于 Nov 30 2012

According to our records, ab9574 was proving difficult to use in western blot and we were in contact in order to help resolve the issue.

Looking at our correspondence, it appears that we are awaiting more details in order to help us better u...

Read More



The non hazardous SDS forms have been placed as 'SDS' PDF files available for download from the online product datasheets for ab9574 and ab87352 on Abcam's website.

Alternatively I have placed links to the PDF files below:

Read More

Thank you for submitting an Abreview for ab9574. As you may have noticed, your review has now been published on our website.
Since you obtained poor results using the antibody in an untested species, we would like to follow up on this to see if we ...

Read More
Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Pig Cell lysate - whole cell (fibroblast)
Loading amount 25 µg
Specification fibroblast
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. jang hoon

Verified customer

提交于 Oct 30 2012

Thank you for the further information.

I am sorry that this antibody is causing problems. Different antibodies work in different optimal conditions, therefore altering some of the experimental conditions for the IGF2 antibody may provide cle...

Read More

1-10 of 34 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"