Anti-IGF1 Receptor (phospho Y1161)抗体(ab85625)


  • 产品名称Anti-IGF1 Receptor (phospho Y1161)抗体
    参阅全部 IGF1 Receptor 一抗
  • 描述
    兔多克隆抗体to IGF1 Receptor (phospho Y1161)
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Chicken, Cow, Drosophila melanogaster
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1150 - 1250 of Human IGF1 Receptor, phosphorylated at Y1161.

    (Peptide available as ab99365.)

  • 阳性对照
    • This antibody gave a positive signal in Human skeletal muscle tissue lysate as well as HEK293 and A549 whole cell lysates.



Our Abpromise guarantee covers the use of ab85625 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 269 kDa (predicted molecular weight: 154 kDa).


  • 功能Receptor tyrosine kinase which mediates actions of insulin-like growth factor 1 (IGF1). Binds IGF1 with high affinity and IGF2 and insulin (INS) with a lower affinity. The activated IGF1R is involved in cell growth and survival control. IGF1R is crucial for tumor transformation and survival of malignant cell. Ligand binding activates the receptor kinase, leading to receptor autophosphorylation, and tyrosines phosphorylation of multiple substrates, that function as signaling adapter proteins including, the insulin-receptor substrates (IRS1/2), Shc and 14-3-3 proteins. Phosphorylation of IRSs proteins lead to the activation of two main signaling pathways: the PI3K-AKT/PKB pathway and the Ras-MAPK pathway. The result of activating the MAPK pathway is increased cellular proliferation, whereas activating the PI3K pathway inhibits apoptosis and stimulates protein synthesis. Phosphorylated IRS1 can activate the 85 kDa regulatory subunit of PI3K (PIK3R1), leading to activation of several downstream substrates, including protein AKT/PKB. AKT phosphorylation, in turn, enhances protein synthesis through mTOR activation and triggers the antiapoptotic effects of IGFIR through phosphorylation and inactivation of BAD. In parallel to PI3K-driven signaling, recruitment of Grb2/SOS by phosphorylated IRS1 or Shc leads to recruitment of Ras and activation of the ras-MAPK pathway. In addition to these two main signaling pathways IGF1R signals also through the Janus kinase/signal transducer and activator of transcription pathway (JAK/STAT). Phosphorylation of JAK proteins can lead to phosphorylation/activation of signal transducers and activators of transcription (STAT) proteins. In particular activation of STAT3, may be essential for the transforming activity of IGF1R. The JAK/STAT pathway activates gene transcription and may be responsible for the transforming activity. JNK kinases can also be activated by the IGF1R. IGF1 exerts inhibiting activities on JNK activation via phosphorylation and inhibition of MAP3K5/ASK1, which is able to directly associate with the IGF1R.
    When present in a hybrid receptor with INSR, binds IGF1. PubMed:12138094 shows that hybrid receptors composed of IGF1R and INSR isoform Long are activated with a high affinity by IGF1, with low affinity by IGF2 and not significantly activated by insulin, and that hybrid receptors composed of IGF1R and INSR isoform Short are activated by IGF1, IGF2 and insulin. In contrast, PubMed:16831875 shows that hybrid receptors composed of IGF1R and INSR isoform Long and hybrid receptors composed of IGF1R and INSR isoform Short have similar binding characteristics, both bind IGF1 and have a low affinity for insulin.
  • 组织特异性Found as a hybrid receptor with INSR in muscle, heart, kidney, adipose tissue, skeletal muscle, hepatoma, fibroblasts, spleen and placenta (at protein level). Expressed in a variety of tissues. Overexpressed in tumors, including melanomas, cancers of the colon, pancreas prostate and kidney.
  • 疾病相关Insulin-like growth factor 1 resistance
  • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
    Contains 4 fibronectin type-III domains.
    Contains 1 protein kinase domain.
  • 翻译后修饰Autophosphorylated on tyrosine residues in response to ligand binding. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit. Autophosphorylation occurs in a sequential manner; Tyr-1165 is predominantly phosphorylated first, followed by phosphorylation of Tyr-1161 and Tyr-1166. While every single phosphorylation increases kinase activity, all three tyrosine residues in the kinase activation loop (Tyr-1165, Tyr-1161 and Tyr-1166) have to be phosphorylated for optimal activity. Can be autophosphorylated at additional tyrosine residues (in vitro). Autophosphorylated is followed by phosphorylation of juxtamembrane tyrosines and C-terminal serines. Phosphorylation of Tyr-980 is required for IRS1- and SHC1-binding. Phosphorylation of Ser-1278 by GSK-3beta restrains kinase activity and promotes cell surface expression, it requires a priming phosphorylation at Ser-1282. Dephosphorylated by PTPN1.
    Polyubiquitinated at Lys-1168 and Lys-1171 through both 'Lys-48' and 'Lys-29' linkages, promoting receptor endocytosis and subsequent degradation by the proteasome. Ubiquitination is facilitated by pre-existing phosphorylation.
    Sumoylated with SUMO1.
    Controlled by regulated intramembrane proteolysis (RIP). Undergoes metalloprotease-dependent constitutive ectodomain shedding to produce a membrane-anchored 52 kDa C-Terminal fragment which is further processed by presenilin gamma-secretase to yield an intracellular 50 kDa fragment.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD221 antibody
    • CD221 antigen antibody
    • IGF 1 receptor antibody
    • IGF 1R antibody
    • IGF I receptor antibody
    • IGF-I receptor antibody
    • Igf1r antibody
    • IGF1R_HUMAN antibody
    • IGFIR antibody
    • IGFIRC antibody
    • IGFR antibody
    • Insulin like growth factor 1 receptor antibody
    • Insulin like growth factor 1 receptor precursor antibody
    • Insulin-like growth factor 1 receptor beta chain antibody
    • Insulin-like growth factor I receptor antibody
    • JTK13 antibody
    • MGC142170 antibody
    • MGC142172 antibody
    • MGC18216 antibody
    • Soluble IGF1R variant 1 antibody
    • Soluble IGF1R variant 2 antibody
    see all

Anti-IGF1 Receptor (phospho Y1161) antibody 图像

  • All lanes : Anti-IGF1 Receptor (phospho Y1161) antibody (ab85625) at 1 µg/ml

    Lane 1 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate with Human IGF1 Receptor (phospho Y1161) peptide (ab99365) at 1 µg/ml
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate with Human IGF1 Receptor (phospho Y1161) peptide (ab99365) at 1 µg/ml
    Lane 3 : Human skeletal muscle tissue lysate - total protein (ab29330) with Human IGF1 Receptor (phospho Y1161) peptide (ab99365) at 1 µg/ml
    Lane 4 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate with Human IGF1 Receptor peptide (ab118178) at 1 µg/ml
    Lane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate with Human IGF1 Receptor peptide (ab118178) at 1 µg/ml
    Lane 6 : Human skeletal muscle tissue lysate - total protein (ab29330) with Human IGF1 Receptor peptide (ab118178) at 1 µg/ml
    Lane 7 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
    Lane 8 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 9 : Human skeletal muscle tissue lysate - total protein (ab29330)

    Lysates/proteins at 10 µg per lane.

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 154 kDa
    Observed band size : 270 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 43 kDa,98 kDa (possible cleavage fragment). We are unsure as to the identity of these extra bands.

    Exposure time : 16 minutes

    IGF1 receptor is exstensively glycosylated (SwissProt) which may explain its migration at a higher molecular weight than predicted. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

  • ICC/IF image of ab85625 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab85625 at 5µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-IGF1 Receptor (phospho Y1161) antibody (ab85625)参考文献

ab85625 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Permeabilization No

Dr. Sophie Pezet

Verified customer

提交于 Feb 14 2012