Anti-IGF1 Receptor beta subunit抗体[1-2] (ab80547)

概述

  • 产品名称Anti-IGF1 Receptor beta subunit抗体[1-2]
  • 描述
    小鼠单克隆抗体[1-2] to IGF1 Receptor beta subunit
  • 经测试应用适用于: WB, IP, Purificationmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse, Chicken, Cow, Xenopus laevis, Zebrafish, Goldfish, Orangutan
  • 免疫原

    Synthetic peptide:

    RKNERALPLPQSSTC

    , corresponding to C terminal amino acids 1323-1337 of Human IGF1 Receptor beta subunit

  • 表位aa 1323-1337
  • 阳性对照
    • Placenta or breast carcinoma.

性能

应用

Our Abpromise guarantee covers the use of ab80547 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 95 kDa.
IP Use at an assay dependent dilution.
Purification Use at an assay dependent dilution.

靶标

  • 功能This receptor binds insulin-like growth factor 1 (IGF1) with a high affinity and IGF2 with a lower affinity. It has a tyrosine-protein kinase activity, which is necessary for the activation of the IGF1-stimulated downstream signaling cascade. When present in a hybrid receptor with INSR, binds IGF1. PubMed:12138094 shows that hybrid receptors composed of IGF1R and INSR isoform Long are activated with a high affinity by IGF1, with low affinity by IGF2 and not significantly activated by insulin, and that hybrid receptors composed of IGF1R and INSR isoform Short are activated by IGF1, IGF2 and insulin. In contrast, PubMed:16831875 shows that hybrid receptors composed of IGF1R and INSR isoform Long and hybrid receptors composed of IGF1R and INSR isoform Short have similar binding characteristics, both bind IGF1 and have a low affinity for insulin.
  • 组织特异性Found as a hybrid receptor with INSR in muscle, heart, kidney, adipose tissue, skeletal muscle, hepatoma, fibroblasts, spleen and placenta (at protein level). Expressed in a variety of tissues.
  • 疾病相关Defects in IGF1R are a cause of insulin-like growth factor 1 resistance (IGF1RES) [MIM:270450]. It is a disorder characterized by intrauterine growth retardation and poor postnatal growth accompanied with increased plasma IGF1.
  • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
    Contains 3 fibronectin type-III domains.
    Contains 1 protein kinase domain.
  • 翻译后修饰The cytoplasmic domain of the beta subunit is autophosphorylated on tyrosine residues in response to insulin-like growth factor I (IGF I).
    Phosphorylation of Tyr-980 is required for IRS1- and SHC1-binding.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CD221 antibody
    • CD221 antigen antibody
    • EC 2.7.10.1 antibody
    • IGF I receptor antibody
    • IGF-I receptor antibody
    • Igf1r antibody
    • IGF1R_HUMAN antibody
    • IGFIR antibody
    • Insulin like growth factor 1 receptor alpha chain antibody
    • Insulin like growth factor 1 receptor beta chain antibody
    • Insulin like growth factor I receptor antibody
    • Insulin-like growth factor 1 receptor beta chain antibody
    • Insulin-like growth factor I receptor antibody
    • JTK13 antibody
    • MGC142170 antibody
    • MGC142172 antibody
    • MGC18216 antibody
    • Soluble IGF1R variant 1 antibody
    • Soluble IGF1R variant 2 antibody
    see all

Anti-IGF1 Receptor beta subunit antibody [1-2] (ab80547)参考文献

This product has been referenced in:
  • Soos MA  et al. Purified hybrid insulin/insulin-like growth factor-I receptors bind insulin-like growth factor-I, but not insulin, with high affinity. Biochem J 290 ( Pt 2):419-26 (1993). WB, AP, IP ; Human, Rat . Read more (PubMed: 8452530) »
  • Soos MA  et al. Immunological studies of type I IGF receptors and insulin receptors: characterisation of hybrid and atypical receptor subtypes. Adv Exp Med Biol 343:145-57 (1993). WB, AP, IP ; Human, Rat . Read more (PubMed: 7514333) »

See all 3 Publications for this product

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Thank you for sending your western blot protocol and the data. I am sorry to see that this antibody is not working for you in this application. Your protocol is correct and the blot with the other antibody demonstrates that the sample is not th...

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Thank you for contacting us and considering the participation in the testing discount scheme. These are the discount codes for the respective antibodies: ab63344 xxxxxxx ab80547 xxxxxxx Both are valid until the xth of xxx 20xx ...

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Thank you for contacting us. We do have one antibody that is specific for IGF1 receptor beta subunit as ab80547. http://www.abcam.com/IGF1-Receptor-beta-subunit-antibody-1-2-BSA-and-Azide-free-ab80547.html We have tested and would...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"