Anti-IGF1 Receptor alpha subunit抗体[24-60] (ab80548)

概述

  • 产品名称Anti-IGF1 Receptor alpha subunit抗体[24-60]
    参阅全部 IGF1 Receptor alpha subunit 一抗
  • 描述
    小鼠单克隆抗体[24-60] to IGF1 Receptor alpha subunit
  • 特异性Weakly reacts with Rabbit and Pig. We have data to indicate that this antibody may not cross react with Rat. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • 经测试应用适用于: IP, Inhibition Assaymore details
    不适用于: WB
  • 种属反应性
    与反应: Rabbit, Human, Pig
  • 免疫原

    IGF-1R/3T3 mouse fibroblasts transfected with human IGF1 Receptor alpha subunit cDNA.

  • 表位aa 184-283
  • 阳性对照
    • Placenta or breast carcinoma
  • 常规说明

    ab80548 acts as a weak IGF-like agonist in Bioassays (Thymidine incorporation into DNA).

性能

应用

Our Abpromise guarantee covers the use of ab80548 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use at 2 µg/mg of lysate. Substantially (>80%) inhibits IGF-1 binding.
Inhibition Assay Use a concentration of 1.5 µg/ml.
  • 应用说明Is unsuitable for WB.
  • 靶标

    • 相关性IGF1 Receptor is a tetramer of 2 alpha and 2 beta chains, linked by disulfide bonds. IGF1 Receptor, like insulin receptor, is synthesized as a single polypeptide which is glycosylated and proteolytically cleaved to give the alpha- and beta-subunits, which are disulfide-linked in a beta-alpha-alpha-beta configuration in the mature receptor. The alpha-subunit is completely extracellular, while the beta-subunit spans the membrane and the intracellular portion has intrinsic tyrosine kinase activity. It is assumed that, as for the insulin receptor, the tyrosine kinase activity is essential for IGF-1 action. The alpha chains contribute to the formation of the ligand-binding domain, while the beta chain carries the kinase domain. It interacts with PIK3R1 and with the PTB/PID domains of IRS1 and SHC1 in vitro when autophosphorylated on tyrosine residues. IGF1 Receptor forms a hybrid receptor with INSR, the hybrid being a tetramer consisting of 1 alpha chain and 1 beta chain of INSR and 1 alpha chain and 1 beta chain of IGF1R.
    • 细胞定位Membrane; Single-pass type I membrane protein.
    • 数据库链接
    • 别名
      • CD221 antibody
      • CD221 antigen antibody
      • IGF I receptor antibody
      • IGFIR antibody
      • Insulin like growth factor I receptor antibody
      • JTK13 antibody
      see all

    Anti-IGF1 Receptor alpha subunit antibody [24-60] (ab80548)参考文献

    This product has been referenced in:
    • Middleton J  et al. Insulin-like growth factor (IGF) receptor, IGF-I, interleukin-1 beta (IL-1 beta), and IL-6 mRNA expression in osteoarthritic and normal human cartilage. J Histochem Cytochem 44:133-41 (1996). Read more (PubMed: 8609369) »
    • Soos MA  et al. Immunological studies of type I IGF receptors and insulin receptors: characterisation of hybrid and atypical receptor subtypes. Adv Exp Med Biol 343:145-57 (1993). Read more (PubMed: 7514333) »

    See all 4 Publications for this product

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