概述

  • 产品名称Anti-IGF1抗体
    参阅全部 IGF1 一抗
  • 描述
    兔多克隆抗体to IGF1
  • 经测试应用适用于: WB, Neutralisingmore details
  • 种属反应性
    与反应: Mouse, Recombinant Fragment
  • 免疫原

    Recombinant murine IGF1

性能

应用

Our Abpromise guarantee covers the use of ab63926 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 4 µg/ml. Detects a band of approximately 9 kDa (predicted molecular weight: 17 kDa).
Neutralising Use a concentration of 2 - 4 µg/ml.

靶标

  • 功能The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake.
  • 疾病相关Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation.
  • 序列相似性Belongs to the insulin family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 形式There are 2 isoforms produced by alternative splicing. Isoform 1 also known as: IGF-IB; Isoform 2 also known as: IGF-IA.
  • 别名
    • IBP1 antibody
    • IGF I antibody
    • IGF IA antibody
    • IGF IB antibody
    • IGF-I antibody
    • Igf1 antibody
    • IGF1_HUMAN antibody
    • IGF1A antibody
    • IGFI antibody
    • IGFIA antibody
    • Insulin like growth factor 1 (somatomedin C) antibody
    • Insulin like growth factor 1 antibody
    • Insulin like growth factor IA antibody
    • Insulin like growth factor IB antibody
    • Insulin-like growth factor I antibody
    • Mechano growth factor antibody
    • MGF antibody
    • OTTHUMP00000195080 antibody
    • OTTHUMP00000195081 antibody
    • OTTHUMP00000195082 antibody
    • OTTHUMP00000195083 antibody
    • OTTHUMP00000195084 antibody
    • Somatomedia C antibody
    • Somatomedin C antibody
    • Somatomedin-C antibody
    see all

Anti-IGF1 antibody 图像

  • All lanes : Anti-IGF1 antibody (ab63926) at 4 µg/ml

    Lane 1 : Recombinant murine IGF1 at 0.25 µg
    Lane 2 : Recombinant murine IGF1 at 1 µg


    Predicted band size : 17 kDa
    Observed band size : 9 kDa (why is the actual band size different from the predicted?)

Anti-IGF1 antibody (ab63926)参考文献

This product has been referenced in:
  • Garcia LA  et al. 1,25(OH)2vitamin D3 stimulates myogenic differentiation by inhibiting cell proliferation and modulating the expression of promyogenic growth factors and myostatin in C2C12 skeletal muscle cells. Endocrinology 152:2976-86 (2011). WB ; Mouse . Read more (PubMed: 21673099) »
  • Mair M  et al. Signal transducer and activator of transcription 3 protects from liver injury and fibrosis in a mouse model of sclerosing cholangitis. Gastroenterology 138:2499-508 (2010). WB . Read more (PubMed: 20193684) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions Reduced Denaturing (15%)
Loading amount 20 µg
Specification Skeletal muscle
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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提交于 Mar 16 2016

Application Western blot
Sample Rat Tissue lysate - whole (Skeletal muscle)
Gel Running Conditions Reduced Denaturing (15%)
Loading amount 20 µg
Specification Skeletal muscle
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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提交于 Mar 16 2016

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (15% Gel)
Sample Rat Tissue lysate - whole (Skeletal muscle)
Specification Skeletal muscle
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jun 12 2014

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (15% Gel)
Sample Mouse Tissue lysate - whole (Skeletal muscle)
Specification Skeletal muscle
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jun 11 2014

The laboratory completed the blocking study for ab177467 (IGF2), and found that the blocking peptide did successfully block the 50 kDa band, indicating that this band is associated with the immunogen.

We found the 2 attached papers, which cite...

Read More

ab63926 has not been tested with mouse skin samples. It seems in serum, IGF1 exists in a large polymeric complex (Refer to the introduction in the attached paper)
“In serum, the majority (70–80%) of the IGFs exist in a 150-kDa complex ...

Read More
Abreviews
Application Western blot
Sample Mouse Tissue lysate - whole (Organ, 293 cell, C2C12 cell)
Loading amount 25 µg
Specification Organ, 293 cell, C2C12 cell
Gel Running Conditions Reduced Denaturing (10% Tris-Tricine gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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提交于 Oct 19 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"