概述

  • 产品名称Anti-IGF1抗体
    参阅全部 IGF1 一抗
  • 描述
    山羊多克隆抗体to IGF1
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Human, Zebrafish
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Dog, Pig
  • 免疫原

    Peptide with sequence

    C-RSVRAQRHTD

    , from the internal region of human IGF1 sequence according to NP_001104753.1; NP_001104754.1; NP_001104755.1; NP_000609.1.

  • 阳性对照
    • Human uterus lysate. This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: HeLa

性能

应用

Our Abpromise guarantee covers the use of ab106836 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 22 kDa.
ICC/IF Use a concentration of 10 µg/ml.

靶标

  • 功能The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake.
  • 疾病相关Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency) [MIM:608747]. IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation.
  • 序列相似性Belongs to the insulin family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 形式There are 2 isoforms produced by alternative splicing. Isoform 1 also known as: IGF-IB; Isoform 2 also known as: IGF-IA.
  • 别名
    • IBP1 antibody
    • IGF I antibody
    • IGF IA antibody
    • IGF IB antibody
    • IGF-I antibody
    • Igf1 antibody
    • IGF1_HUMAN antibody
    • IGF1A antibody
    • IGFI antibody
    • IGFIA antibody
    • Insulin like growth factor 1 (somatomedin C) antibody
    • Insulin like growth factor 1 antibody
    • Insulin like growth factor IA antibody
    • Insulin like growth factor IB antibody
    • Insulin-like growth factor I antibody
    • Mechano growth factor antibody
    • MGF antibody
    • OTTHUMP00000195080 antibody
    • OTTHUMP00000195081 antibody
    • OTTHUMP00000195082 antibody
    • OTTHUMP00000195083 antibody
    • OTTHUMP00000195084 antibody
    • Somatomedia C antibody
    • Somatomedin C antibody
    • Somatomedin-C antibody
    see all

Anti-IGF1 antibody 图像

  • Anti-IGF1 antibody (ab106836) at 0.5 µg/ml + Human uterus lysate at 35 µg
    Developed using the ECL technique

    Predicted band size : 22 kDa
  • ab106836 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab106836 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 donkey anti- goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-IGF1 antibody (ab106836)参考文献

This product has been referenced in:
  • Duran JM  et al. Bone-derived stem cells repair the heart after myocardial infarction through transdifferentiation and paracrine signaling mechanisms. Circ Res 113:539-52 (2013). WB ; Mouse . Read more (PubMed: 23801066) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 100 µg
Gel Running Conditions Non-reduced Non-Denaturing (Native) (12%)
Sample Zebrafish Tissue lysate - whole (muslce (igf1 overexpression in transgenic fish mus)
Specification muslce (igf1 overexpression in transgenic fish mus
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 28°C
Username

Dr. Dongliang Li

Verified customer

提交于 Nov 28 2013

Merci de votre intérêt pour les anti-IGF1 de chez Abcam.

Aucun des anticorps anti-IGF1 Abcam n’a encore été testé à la fois chez l'homme, la souris et le rat et nous n’offrons pas d’&e...

Read More

Thank you for contacting us. We can recomend using Tris triton or RIPA buffer for lysates preparation. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"