概述

  • 产品名称Anti-IgE抗体[5D4]
    参阅全部 IgE 一抗
  • 描述
    小鼠单克隆抗体[5D4] to IgE
  • 特异性Specific for IgE Ce2-domain specific
  • 经测试应用适用于: ELISA, Sandwich ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Full length native protein (purified) corresponding to Human IgE. IgE from myelomatous human serum.

  • 常规说明Concentration varies from lot to lot and can be provided on request.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度Protein A purified
  • 纯化说明Purified by chromatography on protein A Sepharose. Purity is tested by electrophoresis.
  • 克隆单克隆
  • 克隆编号5D4
  • 骨髓瘤Sp2/0
  • 同种型IgG2a
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab7295 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent dilution. Ab7295 can be used for the detection of total IgE levels and IgE antibodies by ELISA
Sandwich ELISA Use at an assay dependent dilution. Can be paired for Sandwich ELISA with Mouse monoclonal [4F4] to IgE (HRP) (ab7294). Can be used as Capture antibody with recommended pair.

靶标

  • 相关性IgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect against parasite invasion, but it is a major factor in allergic reactions. The antigen-specific IgE interacts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and other substances that lead to the itching, sneezing and congestion of allergies - and the life threatening respiratory distress of asthma and anaphylactic shock.
  • 细胞定位Cell Membrane
  • 数据库链接
  • 别名
    • Igh2 antibody
    • IGHE antibody
    • IGHEP1 antibody
    • Immunoglobulin heavy constant epsilon antibody

Anti-IgE antibody [5D4] 图像

  • Standard curve: Capture anitbody (ab7295) at 5 ug/ml in PBS, human IgE from 100 ng/ml to 1.56 ng/ml, detection antibody (ab7294, HRP conjugated) at 0.7 ug/ml concentration.

Anti-IgE antibody [5D4] (ab7295)参考文献

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See 1 Publication for this product

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Thank you for contacting us.   I have received the following information from the lab: We are using ab7295 according to the datasheet recommendation as the capture antibody, not as detection antibody. The detection antibody ab7295 was used with the...

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Thank you for your enquiry. In our internal assay for anti-IgE MAb testing, highly purified myelomatous IgE(VL) was adsorbed on solid phase from solution (5 microgram/ml) in bicarbonate buffer pH 9.5. Ab7295 was applied for 60-120 min onto antige...

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Thank you for your enquiry and for completing our technical questionnaire. I am sorry to hear that you have been having difficulties with this antibody. I would greatly appreciate it if you could tell me of the concentration that you have been using...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"