概述

  • 产品名称Anti-IFT43抗体
  • 描述
    兔多克隆抗体to IFT43
  • 经测试应用适用于: ICC/IF, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 107-202 (EEVQEEDFVL QVAAPPSIQI KRVMTYRDLD NDLMKYSAIQ TLDGEIDLKL LTKVLAPEHE VREDDVGWDW DHLFTEVSSE VLTEWDPLQT EKEDPA) of Human IFT43 (Q96FT9).

  • 阳性对照
    • IFT43-transfected HEK293T lysate; Human testis tissue.

性能

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应用

Our Abpromise guarantee covers the use of ab121195 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

靶标

  • 功能Component of IFT complex A (IFT-A) involved in retrograde ciliary transport along microtubules from the ciliary tip to the base.
  • 疾病相关Defects in IFT43 are a cause of cranioectodermal dysplasia type 1 (CED1) [MIM:218330]. CED1 is a disorder characterized by craniofacial, skeletal and ectodermal abnormalities. Clinical features include dolichocephaly (with or without sagittal suture synostosis), scaphocephaly, short stature, limb shortening, short ribs, narrow chest, brachydactyly, renal failure and hepatic fibrosis, small and abnormally shaped teeth, sparse hair, skin laxity and abnormal nails.
  • 序列相似性Belongs to the IFT43 family.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C14orf179 antibody
    • ift43 antibody
    • IFT43_HUMAN antibody
    • Intraflagellar transport protein 43 homolog antibody

Anti-IFT43 antibody 图像

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in cytoskeleton. Recommended concentration of ab121195 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • All lanes : Anti-IFT43 antibody (ab121195) at 1/250 dilution

    Lane 1 : Vector only transfected HEK293T lysate
    Lane 2 : IFT43-transfected HEK293T lysate (Co-expressed with a C-terminal myc-DDK tag ~3.1 kDa)

    Developed using the ECL technique
  • ab121195 at 1/50 dilution staining IFT43 in Paraffin-embedded Human testis tissue by Immunohistochemistry.

Anti-IFT43 antibody (ab121195)参考文献

ab121195 has not yet been referenced specifically in any publications.

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