概述

  • 产品名称
  • 描述
    小鼠多克隆抗体to IFT20
  • 特异性
    Reactive against mammalian transfected lysate.
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    IFT20 (NP_777547, 1 a.a. ~ 148 a.a) full-length human protein.

  • 阳性对照
    • Transfected 293T golgi apparatus lysate

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab73110 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明
    WB: 1/500 - 1/1000. Detects a band of approximately 16 kDa.

    Detection antibody.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性
      Eukaryotic cilia are assembled via intraflagellar transport (IFT) in which large protein particles are motored along ciliary microtubules. The IFT20 subunit of a particle is localized to the Golgi complex, plus the basal body and cilia. In living cells, fluorescently tagged IFT20 is highly dynamic and moves between the Golgi complex and the cilium as well as along ciliary microtubules. It has been suggested that IFT20 functions in the delivery of ciliary membrane proteins from the Golgi complex to the cilium. There are three different isoforms.
    • 细胞定位
      Golgi apparatus. Cilium. Centrosome, centriole.
    • 数据库链接
    • 别名
      • hIFT20 antibody
      • Intraflagellar transport 20 homolog (Chlamydomonas) antibody
      • Intraflagellar transport protein 20 like antibody
      • Intraflagellar transport protein IFT20 antibody

    Anti-IFT20 antibody 图像

    • All lanes : Anti-IFT20 antibody (ab73110) at 1/500 dilution

      Lane 1 : IFT20 transfected 293T cell line
      Lane 2 : Non transfected 293T cell line

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Observed band size : 16 kDa (why is the actual band size different from the predicted?)

    实验方案

    Anti-IFT20 antibody (ab73110)参考文献

    This product has been referenced in:
    • Mandell MA  et al. TRIM17 contributes to autophagy of midbodies while actively sparing other targets from degradation. J Cell Sci 129:3562-3573 (2016). Read more (PubMed: 27562068) »

    See 1 Publication for this product

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