概述

  • 产品名称Anti-IFT20抗体
    参阅全部 IFT20 一抗
  • 描述
    小鼠多克隆抗体to IFT20
  • 特异性Reactive against mammalian transfected lysate.
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    IFT20 (NP_777547, 1 a.a. ~ 148 a.a) full-length human protein.

  • 阳性对照
    • Transfected 293T golgi apparatus lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab73110 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: 1/500 - 1/1000. Detects a band of approximately 16 kDa.

    Detection antibody.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性Eukaryotic cilia are assembled via intraflagellar transport (IFT) in which large protein particles are motored along ciliary microtubules. The IFT20 subunit of a particle is localized to the Golgi complex, plus the basal body and cilia. In living cells, fluorescently tagged IFT20 is highly dynamic and moves between the Golgi complex and the cilium as well as along ciliary microtubules. It has been suggested that IFT20 functions in the delivery of ciliary membrane proteins from the Golgi complex to the cilium. There are three different isoforms.
    • 细胞定位Golgi apparatus. Cilium. Centrosome, centriole.
    • 数据库链接
    • 别名
      • hIFT20 antibody
      • Intraflagellar transport 20 homolog (Chlamydomonas) antibody
      • Intraflagellar transport protein 20 like antibody
      • Intraflagellar transport protein IFT20 antibody

    Anti-IFT20 antibody 图像

    • All lanes : Anti-IFT20 antibody (ab73110) at 1/500 dilution

      Lane 1 : IFT20 transfected 293T cell line
      Lane 2 : Non transfected 293T cell line

      Lysates/proteins at 25 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Observed band size : 16 kDa (why is the actual band size different from the predicted?)

    Anti-IFT20 antibody (ab73110)参考文献

    This product has been referenced in:
    • Mandell MA  et al. TRIM17 contributes to autophagy of midbodies while actively sparing other targets from degradation. J Cell Sci 129:3562-3573 (2016). Read more (PubMed: 27562068) »

    See 1 Publication for this product

    Product Wall

    There are currently no Abreviews or Questions for ab73110.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"