概述

  • 产品名称Anti-IFT20抗体
    参阅全部 IFT20 一抗
  • 描述
    兔多克隆抗体to IFT20
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 50-80 of Human IFT20, conjugated to KLH.

  • 阳性对照
    • Mouse lung tissue lysates; Human hepatocarcinoma tissue This antibody gave a positive result when used in the following methanol fixed cell lines: WI 38

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • 存储溶液Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab103693 is purified through a protein A column, followed by peptide affinity purification.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab103693 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/100 - 1/500. Predicted molecular weight: 15 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 相关性Eukaryotic cilia are assembled via intraflagellar transport (IFT) in which large protein particles are motored along ciliary microtubules. The IFT20 subunit of a particle is localized to the Golgi complex, plus the basal body and cilia. In living cells, fluorescently tagged IFT20 is highly dynamic and moves between the Golgi complex and the cilium as well as along ciliary microtubules. It has been suggested that IFT20 functions in the delivery of ciliary membrane proteins from the Golgi complex to the cilium. There are three different isoforms.
  • 细胞定位Golgi apparatus. Cilium. Centrosome, centriole.
  • 数据库链接
  • 别名
    • hIFT20 antibody
    • Intraflagellar transport 20 homolog (Chlamydomonas) antibody
    • Intraflagellar transport protein 20 like antibody
    • Intraflagellar transport protein IFT20 antibody

Anti-IFT20 antibody 图像

  • ab103693 stained WI 38 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab103693 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-IFT20 antibody (ab103693) at 1/100 dilution + Mouse lung tissue lysates at 35 µg

    Predicted band size : 15 kDa
  • ab103693, at a 1/50 dilution, staining IFT20 in formalin fixed, paraffin embedded Human hepatocarcinoma tissue by Immunohistochemistry, followed by peroxidase conjugated secondary antibody and DAB staining.

Anti-IFT20 antibody (ab103693)参考文献

ab103693 has not yet been referenced specifically in any publications.

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