概述

  • 产品名称Anti-IFT140抗体
  • 描述
    兔多克隆抗体to IFT140
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat
  • 免疫原

    Synthetic peptide corresponding to N terminal amino acids 108-157 (VLRWSPSGNC LLSGDRLGVL LLWRLDQRGR VQGTPLLKHE YGKHLTHCIF) of human IFT140 (NP_055529).

  • 阳性对照
    • Fetal brain lysate

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明ab83183 is purified by peptide affinity chromatography method.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab83183 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 165 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:62500.

靶标

  • 相关性IFT140 contains 9 TPR repeats and 5 WD repeats. The function of the IFT140 protein remains unknown.
  • 数据库链接
  • 别名
    • Gs114 antibody
    • Intraflagellar transport 140 homolog (Chlamydomonas) antibody
    • WD and tetratricopeptide repeats protein 2 antibody
    • WDTC2 antibody

Anti-IFT140 antibody 图像

  • Anti-IFT140 antibody (ab83183) at 1 µg/ml (in 5% skim milk / PBS buffer) + fetal brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 165 kDa
    Observed band size : 165 kDa
    Additional bands at : 150 kDa. We are unsure as to the identity of these extra bands.

Anti-IFT140 antibody (ab83183)参考文献

ab83183 has not yet been referenced specifically in any publications.

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