Anti-IFRD1抗体(ab81646)

概述

  • 产品名称Anti-IFRD1抗体
    参阅全部 IFRD1 一抗
  • 描述
    兔多克隆抗体to IFRD1
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • 免疫原

    Synthetic peptide: LALLFELARG IESDFFYEDM ESLTQMLRAL ATDGNKHRAK VDKRKQRSVF , corresponding to N terminal amino acids 290-339 of Human IFRD1 (NP_001541).

  • 阳性对照
    • Fetal heart lysate

性能

应用

Our Abpromise guarantee covers the use of ab81646 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 50 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:312500.

靶标

Anti-IFRD1 antibody 图像



  • Predicted band size : 50 kDa
    Western blot analysis of Human fetal lung tissue lysate labeling IFRD1 with ab81646 at 1.0µg/ml.
  • Anti-IFRD1 antibody (ab81646) at 1 µg/ml + Fetal heart lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 50 kDa
    Observed band size : 50 kDa

Anti-IFRD1 antibody (ab81646)参考文献

ab81646 has not yet been referenced specifically in any publications.

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Thank you for your patience. I have confirmed with the lab that the fetal heart lysate was from human species. Regarding how to process the tissue lysate, please refer to our protocols page: A. Sample preparation 4. Preparation of lysate from t...

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