概述

  • 产品名称Anti-IFNAR2抗体
    参阅全部 IFNAR2 一抗
  • 描述
    兔多克隆抗体to IFNAR2
  • 经测试应用适用于: ICC/IF, WB, ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic peptide corresponding to C terminal residues of human IFNAR2 (Interferon-alpha/beta receptor beta chain precursor)

性能

应用

Our Abpromise guarantee covers the use of ab56070 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 58 kDa. Two hour incubation. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
ELISA Use at an assay dependent dilution.

靶标

  • 功能Associates with IFNAR1 to form the type I interferon receptor. Receptor for interferons alpha and beta. Involved in IFN-mediated STAT1, STAT2 and STAT3 activation. Isoform 1 and isoform 2 are directly involved in signal transduction due to their association with the TYR kinase, JAK1. Isoform 3 is a potent inhibitor of type I IFN receptor activity.
  • 组织特异性Isoform 3 is detected in the urine (at protein level). Expressed in blood cells. Expressed in lymphoblastoid and fibrosarcoma cell lines.
  • 序列相似性Belongs to the type II cytokine receptor family.
  • 翻译后修饰Phosphorylated on tyrosine residues upon interferon binding. Phosphorylation at Tyr-337 or Tyr-512 are sufficient to mediate interferon dependent activation of STAT1, STAT2 and STAT3 leading to antiproliferative effects on many different cell types.
    Glycosylated.
  • 细胞定位Secreted and Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Human interferon alpha/beta receptor antibody
    • IFN alpha REC antibody
    • IFN R antibody
    • IFN-a/ßR2 antibody
    • IFN-a/ßRß antibody
    • IFN-alpha binding protein antibody
    • IFN-alpha/beta receptor 2 antibody
    • IFN-R-2 antibody
    • IFNABR antibody
    • IFNAR2 antibody
    • IFNARB antibody
    • IFNR antibody
    • INAR2_HUMAN antibody
    • Interferon (alpha beta and omega) receptor 2 antibody
    • Interferon alpha binding protein antibody
    • Interferon alpha/beta receptor 2 antibody
    • Interferon alpha/beta receptor beta chain antibody
    • Type I interferon receptor 2 antibody
    • Type I interferon receptor antibody
    see all

Anti-IFNAR2 antibody 图像

  • ICC/IF image of ab56070 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56070, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-IFNAR2 antibody (ab56070)参考文献

This product has been referenced in:
  • Happold C  et al. Interferon-ß induces loss of spherogenicity and overcomes therapy resistance of glioblastoma stem cells. Mol Cancer Ther 13:948-61 (2014). Read more (PubMed: 24526161) »
  • Hong XX & Carmichael GG Innate immunity in pluripotent human cells: attenuated response to interferon-ß. J Biol Chem 288:16196-205 (2013). Human . Read more (PubMed: 23599426) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Lung)
Specification Lung
Fixative Paraformaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 23°C
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提交于 Jun 30 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"