重组
RabMAb

Anti-IFNAR1抗体[EP899Y] (ab45172)

概述

  • 产品名称
    Anti-IFNAR1抗体[EP899Y]
    参阅全部 IFNAR1 一抗
  • 描述
    兔单克隆抗体[EP899Y] to IFNAR1
  • 特异性
    This antibody is specific for the 90kda form and the full-length form of IFNAR1 protein.
  • 经测试应用
    适用于: WB, Flow Cyt, IPmore details
    不适用于: IHC-P
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to residues near the C-terminus of human IFNAR1.

  • 阳性对照
    • Permeabilised K562 cells, K562 cell lysate.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

应用

Our Abpromise guarantee covers the use of ab45172 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 90,130 kDa (predicted molecular weight: 64 kDa). Due to extensive glycosylation of the mature molecule; the 90 kDa species is a minor incompletely processed species.
Flow Cyt 1/30. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
IP 1/50.
  • 应用说明
    Is unsuitable for IHC-P.
  • 靶标

    • 功能
      Associates with IFNAR2 to form the type I interferon receptor. Receptor for interferons alpha and beta. Binding to type I IFNs triggers tyrosine phosphorylation of a number of proteins including JAKs, TYK2, STAT proteins and IFNR alpha- and beta-subunits themselves.
    • 组织特异性
      IFN receptors are present in all tissues and even on the surface of most IFN-resistant cells. Isoform 1, isoform 2 and isoform 3 are expressed in the IFN-alpha sensitive myeloma cell line U266S. Isoform 2 and isoform 3 are expressed in the IFN-alpha resistant myeloma cell line U266R. Isoform 1 is not expressed in IFN-alpha resistant myeloma cell line U266R.
    • 序列相似性
      Belongs to the type II cytokine receptor family.
      Contains 3 fibronectin type-III domains.
    • 翻译后修饰
      Phosphorylated on tyrosine residues by TYK2 tyrosine kinase.
    • 细胞定位
      Membrane.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Alpha type antiviral protein antibody
      • Antiviral protein, alpha-type antibody
      • Antiviral protein, beta-type antibody
      • AVP antibody
      • Beta type antiviral protein antibody
      • CRF2-1 antibody
      • Cytokine receptor class-II member 1 antibody
      • Cytokine receptor family 2 member 1 antibody
      • IFN alpha REC antibody
      • IFN alpha receptor antibody
      • IFN alpha/beta Receptor alpha antibody
      • IFN beta receptor antibody
      • IFN Interferon-beta receptor antibody
      • IFN-alpha/beta receptor 1 antibody
      • IFN-R-1 antibody
      • IFNAR antibody
      • Ifnar1 antibody
      • IFNBR antibody
      • IFRC antibody
      • INAR1_HUMAN antibody
      • Interferon (alpha beta and omega) receptor 1 antibody
      • interferon alpha and beta receptor subunit 1 antibody
      • Interferon alpha/beta receptor 1 antibody
      • Interferon alpha/beta receptor alpha chain antibody
      • Interferon beta receptor 1 antibody
      • interferon receptor 1 antibody
      • Interferon-alpha receptor antibody
      • Type I interferon receptor 1 antibody
      see all

    图片

    • Anti-IFNAR1 antibody [EP899Y] (ab45172) at 1/20000 dilution + K562 cell lysate at 10 µg

      Secondary
      Goat anti rabbit HRP labelled. at 1/2000 dilution

      Predicted band size : 64 kDa
      Observed band size : 90,130 kDa (why is the actual band size different from the predicted?)
    • Flow cytometric analysis of permeabilized K562 cells using ab45172 at a 1/30 dilution (red) or a rabbit IgG (negative) (green).

    文献

    This product has been referenced in:
    • Tian J  et al. Blocking the PI3K/AKT pathway enhances mammalian reovirus replication by repressing IFN-stimulated genes. Front Microbiol 6:886 (2015). Read more (PubMed: 26388843) »
    • Chandra PK  et al. Impaired expression of type I and type II interferon receptors in HCV-associated chronic liver disease and liver cirrhosis. PLoS One 9:e108616 (2014). Read more (PubMed: 25265476) »

    See all 10 Publications for this product

    客户评价及客户问答

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Flow Cytometry
    Fixation
    BD #554722
    Sample
    Human Cell (primary human macrophages)
    Specification
    primary human macrophages
    Gating Strategy
    macrophages
    Permeabilization
    No
    Username

    Abcam user community

    Verified customer

    提交于 Jul 19 2013

    Thank you for contacting us.

    I would recommend you consult the manufacturer of the beads which you intent to use. The binding capacity will be determined by the manufacturer of the beads. For example Millipore'sPureProteome Protein A and P...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (vascular smooth muscle cells)
    Loading amount
    10 µg
    Specification
    vascular smooth muscle cells
    Treatment
    DTT and 5 min boiling
    Gel Running Conditions
    Reduced Denaturing (4-12 % gradients)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    提交于 Apr 27 2012

    Thank you for your enquiry. A general protocol for intracellular staining with this antibody can be found at the following link: http://www.abcam.com/ps/pdf/protocols/flow_intracellular_staining.pdf A second protocol describes indirect stainin...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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